Boyce W M, Mazet J A, Mellies J, Gardner I, Clark R K, Jessup D A
Department of Veterinary Microbiology and Immunology, University of California, Davis 95616.
J Parasitol. 1991 Oct;77(5):692-6.
A kinetic enzyme-linked immunosorbent assay (ELISA) was developed using antigenic extracts prepared from Psoroptes cuniculi mites and sera from 37 Psoroptes sp.-infested and 43 uninfested bighorn sheep (Ovis canadensis). Serial dilutions of these serum samples, representing 3 bighorn sheep subspecies and 9 geographic areas, gave parallel responses when plotted as log dilution versus log kinetic rate. Therefore, all 80 samples were run at a single dilution (1:100) and positive/negative cutoff values were established as the mean kinetic rate of all negative sera plus either 2, 3, or 4 standard deviations. The resulting ELISA was highly reproducible and accurate with sensitivities and specificities of 100% and 97.7%, 94.6% and 97.7%, and 94.6% and 100%, respectively. This immunoassay will be useful for prospective and retrospective studies assessing the distribution and prevalence of Psoroptes sp. infestations in bighorn sheep.
利用从兔痒螨制备的抗原提取物以及来自37只感染痒螨属螨类的大角羊和43只未感染的大角羊（加拿大盘羊）的血清，开发了一种动力学酶联免疫吸附测定（ELISA）。这些血清样本代表3个大角羊亚种和9个地理区域，将其系列稀释液以对数稀释度对对数动力学速率作图时，呈现出平行反应。因此，所有80个样本均以单一稀释度（1:100）进行检测，并将阳性/阴性临界值设定为所有阴性血清的平均动力学速率加上2、3或4个标准差。所得的ELISA具有高度可重复性和准确性，敏感性和特异性分别为100%和97.7%、94.6%和97.7%以及94.6%和100%。这种免疫测定法将有助于进行前瞻性和回顾性研究，以评估大角羊中痒螨属螨类感染的分布和流行情况。
