Boyce W M, Jessup D A, Clark R K
Department of Veterinary Microbiology and Immunology, University of California, Davis 95616.
J Wildl Dis. 1991 Jan;27(1):10-5. doi: 10.7589/0090-3558-27.1.10.
The antibody responses of bighorn sheep (Ovis canadensis) infected with Psoroptes sp. mites were investigated by enzyme linked immunosorbent assay on western blots of P. cuniculi antigens. Serum from 20 Psoroptes sp.-infested bighorn sheep (O. canadensis mexicana, O. canadensis nelsoni, O. canadensis canadensis) from New Mexico, Nevada, California, and Idaho reacted strongly with mite antigens ranging from 12 to 34 kd. Serum from 35 Psoroptes sp.-free bighorn sheep of unknown tick infestation status and from three Psoroptes sp.-free bighorn sheep infested with Dermacentor hunteri ticks did not react with these antigens. Psoroptes sp.-specific antibody responses were present throughout a 16 mo period in one infected bighorn sheep, but were not detectable 8 mo following successful treatment. These results demonstrate that specific serodiagnosis of Psoroptes sp. infestation is feasible in bighorn sheep and suggest that antibody responses are indicative of current or recent infestation.
通过酶联免疫吸附测定法，在兔痒螨抗原的蛋白质印迹上，研究了感染疥螨属螨虫的大角羊（加拿大盘羊）的抗体反应。来自新墨西哥州、内华达州、加利福尼亚州和爱达荷州的20只感染疥螨属螨虫的大角羊（墨西哥加拿大盘羊、尼尔森加拿大盘羊、加拿大加拿大盘羊）的血清，与分子量在12至34kd之间的螨虫抗原发生强烈反应。来自35只未知蜱虫感染状况的未感染疥螨属螨虫的大角羊以及3只感染亨特革蜱的未感染疥螨属螨虫的大角羊的血清，未与这些抗原发生反应。在一只感染的大角羊中，整个16个月期间均存在疥螨属特异性抗体反应，但在成功治疗8个月后无法检测到。这些结果表明，在大角羊中对疥螨属感染进行特异性血清诊断是可行的，并表明抗体反应表明存在当前或近期感染。
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