Maki J, Weinstein P P
Department of Biological Sciences, University of Notre Dame, Indiana 46556.
J Parasitol. 1991 Oct;77(5):749-54.
The reproductive integrity and viability of adult female Acanthocheilonema viteae (syn. Dipetalonema viteae) maintained in culture for relatively long periods were assessed by transplantation into jirds. Worms cultured in chemically defined NI medium for approximately 3-4 wk remained active, but microfilarial release declined to barely detectable levels. Microfilarial production, however, was restored when the worms were transplanted subcutaneously into jirds. When cultured in NI medium beyond 4 wk no restoration of microfilarial production occurred on transplantation, presumably due to irreversible injury to the reproductive system. However, when NI medium was supplemented with fetal bovine serum resumption of microfilarial production occurred in transplanted females that had been in culture for as long as 2 1/2 mo. The addition of serum to NI medium played an important role in maintaining and protecting the functional integrity of the reproductive system.
通过移植到沙鼠体内，评估了在培养中维持较长时间的成年雌性旋盘尾丝虫（同义词：罗阿罗阿丝虫）的生殖完整性和活力。在化学成分明确的NI培养基中培养约3 - 4周的蠕虫仍保持活跃，但微丝蚴释放量降至几乎检测不到的水平。然而，当将这些蠕虫皮下移植到沙鼠体内时，微丝蚴的产生得以恢复。当在NI培养基中培养超过4周时，移植后微丝蚴的产生没有恢复，推测是由于生殖系统受到不可逆损伤。然而，当NI培养基中添加胎牛血清时，在培养长达2个半月的移植雌性蠕虫中出现了微丝蚴产生的恢复。向NI培养基中添加血清在维持和保护生殖系统的功能完整性方面发挥了重要作用。
