Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Atlanta, Georgia 30341, USA.
Anal Chem. 2009 Mar 1;81(5):1931-6. doi: 10.1021/ac802419n.
Because of health concerns surrounding in utero and neonatal exposure to perchlorate, we developed a method for analyzing perchlorate in the dried blood spots (DBS) of newborns. Ion chromatography was interfaced with electrospray ionization tandem mass spectrometry to measure blood perchlorate levels in DBS samples as low as 0.10 ng/mL. Measurement of perchlorate in DBS indicated good precision (relative standard deviations ranging from 5.8% to 16.2%) and accuracy (% difference values ranging from -11.3% to -12.1%). Perchlorate was detectable in 100% of the DBS collected from 100 newborns. These samples had a median blank-adjusted concentration of 1.88 ng/mL. Such data support the utility of this method to quantify perchlorate levels in DBS samples. Applying this method to analyze neonatal DBS will improve perchlorate exposure assessments of this susceptible population.
由于担心孕妇和新生儿会接触到高氯酸盐，我们开发了一种方法来分析新生儿干血斑（DBS）中的高氯酸盐。离子色谱法与电喷雾串联质谱联用，可测量 DBS 样本中低至 0.10ng/mL 的血高氯酸盐水平。DBS 中高氯酸盐的测量具有良好的精密度（相对标准偏差范围为 5.8%至 16.2%）和准确度（%差值范围为-11.3%至-12.1%）。在从 100 名新生儿中采集的 100%的 DBS 中均可检测到高氯酸盐。这些样本的中位空白调整浓度为 1.88ng/mL。这些数据支持该方法可用于定量 DBS 样本中高氯酸盐水平的实用性。应用该方法分析新生儿 DBS 将改善对这一易感人群的高氯酸盐暴露评估。
