亲水作用色谱-串联质谱法分析奶粉及牛奶中的高氯酸盐

Analysis of perchlorate in milk powder and milk by hydrophilic interaction chromatography combined with tandem mass spectrometry.

机构信息

Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, RP China.

出版信息

J Agric Food Chem. 2010 Mar 24;58(6):3736-40. doi: 10.1021/jf903801m.

DOI:10.1021/jf903801m

PMID:20170169

Abstract

A simple, selective, and sensitive method using hydrophilic interaction chromatography combined with tandem mass spectrometry (HILIC-MS/MS) for quantifying perchlorate in milk powder and milk was developed. The analysis was conducted on an Inertsil HILIC column (150 mm x 3.0 mm, 3.5 mum) using a mobile phase consisting of methanol and 0.1% formic acid (60:40, v/v). The detection was performed by MS/MS via electrospray ionization. Linear calibration curves were obtained in the concentration range of 2.00 x 10(-2) to 8.00 microg/g and 4.00 x 10(-1) to 20.0 microg/L for perchlorate in milk powder and milk, respectively. The method detection limit was 4.00 x 10(-3) microg/g for milk powder and 8.00 x 10(-2) microg/L for milk. The recoveries of perchlorate in milk powder and milk were all >90%. This method was successfully applied to the quantitative determination of perchlorate in milk powder and milk.

摘要

建立了一种利用亲水作用色谱-串联质谱（HILIC-MS/MS）测定奶粉和牛奶中高氯酸盐的简单、选择性和灵敏的方法。分析在 Inertsil HILIC 柱（150mm×3.0mm，3.5μm）上进行，流动相由甲醇和 0.1%甲酸（60:40，v/v）组成。检测采用电喷雾电离的 MS/MS 进行。奶粉和牛奶中高氯酸盐的线性校准曲线范围分别为 2.00×10(-2)至 8.00μg/g 和 4.00×10(-1)至 20.0μg/L。方法检测限为奶粉中 4.00×10(-3)μg/g，牛奶中 8.00×10(-2)μg/L。奶粉和牛奶中高氯酸盐的回收率均>90%。该方法成功应用于奶粉和牛奶中高氯酸盐的定量测定。