Barksdale E M, McGenis T G, Donahoe P K
Department of Surgery, Massachusetts General Hospital, Boston 02114.
J Pediatr Surg. 1991 Aug;26(8):886-92. doi: 10.1016/0022-3468(91)90831-d.
Factors that favor graft survival of fetal and neonatal testis relative to adult testis were explored by studying the effects of rapid growth on immunogenicity. Tissue-specific growth was initiated by elevated gonadotropins created by oophorectomy and allografted target testes were examined. Three-day postnatal testes were implanted under the subrenal capsule of oophorectomized (as confirmed by elevated gonadotropins) and nonoophorectomized females. Immunosuppressive therapy with cyclosporine A was administered to selected animals of both groups. Preliminary studies in outbred rats and more extensive studies in allogenic/congenic mice (C57BL/6J to B10.A) showed that testicular allografts exposed to the elevated gonadotropins caused by previous host oophorectomy grow larger, have less lymphocytic infiltrate, and show better preservation of architecture than do allografts in nonoophorectomized female recipients. The graft survival resulting in vivo from elevated gonadotropins approximated that permitted by either maximal immunosuppression or syngeneic transplantation.
通过研究快速生长对免疫原性的影响，探索了相对于成年睾丸而言有利于胎儿和新生儿睾丸移植物存活的因素。通过卵巢切除所产生的促性腺激素升高引发组织特异性生长，并对同种异体移植的目标睾丸进行检查。将出生后三天的睾丸植入经卵巢切除（通过促性腺激素升高确认）和未经卵巢切除的雌性动物的肾下囊。对两组中的选定动物给予环孢素A免疫抑制治疗。在远交系大鼠中的初步研究以及在同种异体/同基因小鼠（C57BL/6J至B10.A）中的更广泛研究表明，与未经卵巢切除的雌性受体中的同种异体移植物相比，暴露于先前宿主卵巢切除所导致的促性腺激素升高的睾丸同种异体移植物生长更大，淋巴细胞浸润更少，并且结构保存更好。促性腺激素升高在体内导致的移植物存活近似于最大免疫抑制或同基因移植所允许的情况。
