U.S. Geological Survey, Ohio Water Science Center, Columbus, OH, USA.
J Appl Microbiol. 2009 Feb;106(2):432-41. doi: 10.1111/j.1365-2672.2008.04011.x.
The aim of this study was to examine a rapid method for detecting Escherichia coli and enterococci in recreational water.
Water samples were assayed for E. coli and enterococci by traditional and immunomagnetic separation/adenosine triphosphate (IMS/ATP) methods. Three sample treatments were evaluated for the IMS/ATP method: double filtration, single filtration, and direct analysis. Pearson's correlation analysis showed strong, significant, linear relations between IMS/ATP and traditional methods for all sample treatments; strongest linear correlations were with the direct analysis (r = 0.62 and 0.77 for E. coli and enterococci, respectively). Additionally, simple linear regression was used to estimate bacteria concentrations as a function of IMS/ATP results. The correct classification of water-quality criteria was 67% for E. coli and 80% for enterococci.
The IMS/ATP method is a viable alternative to traditional methods for faecal-indicator bacteria.
The IMS/ATP method addresses critical public health needs for the rapid detection of faecal-indicator contamination and has potential for satisfying US legislative mandates requiring methods to detect bathing water contamination in 2 h or less. Moreover, IMS/ATP equipment is considerably less costly and more portable than that for molecular methods, making the method suitable for field applications.
本研究旨在检验一种快速检测娱乐水中大肠杆菌和肠球菌的方法。
采用传统免疫磁分离/三磷酸腺苷（IMS/ATP）法检测水样中的大肠杆菌和肠球菌。评估了 IMS/ATP 方法的三种样品处理方法：双重过滤、单一过滤和直接分析。Pearson 相关性分析表明，对于所有样品处理方法，IMS/ATP 与传统方法之间存在强、显著、线性关系；与直接分析的线性相关性最强（大肠杆菌和肠球菌的 r 值分别为 0.62 和 0.77）。此外，还使用简单线性回归来估计细菌浓度作为 IMS/ATP 结果的函数。对于大肠杆菌，水质标准的正确分类为 67%；对于肠球菌，正确分类为 80%。
IMS/ATP 法是粪便指示菌传统检测方法的可行替代方法。
IMS/ATP 方法满足了快速检测粪便指示物污染的迫切公共卫生需求，并有可能满足美国立法要求，即要求在 2 小时或更短时间内检测浴场水的污染。此外，IMS/ATP 设备的成本明显低于分子方法，且更便于携带，因此非常适合现场应用。
