使用Dynabeads蛋白G偶联物从生菜和牛奶中高效、特异性地分离 。 你提供的原文似乎不完整，“from lettuce and milk using Dynabeads protein G conjugates.”前面应该还有具体要分离的物质。
Highly efficient and specific separation of from lettuce and milk using Dynabeads protein G conjugates.
作者信息
Wei Shuai, Park Byung-Jae, Seo Kun-Ho, Oh Deog-Hwan
机构信息
1Department of Food Science and Biotechnology, School of Bioconvergence Science and Technology, Kangwon National University, Chuncheon, Gangwon, 24341 Korea.
2KU Center for Food Safety, College of Veterinary Medicine, Konkuk University, Seoul, 05029 Korea.
出版信息
Food Sci Biotechnol. 2016 Oct 31;25(5):1501-1505. doi: 10.1007/s10068-016-0233-1. eCollection 2016.
An immunomagnetic separation method using antibody-coated Dynabeads Protein G was developed for specific and efficient separation of in lettuce and whole milk. The amount of immunomagnetic beads (IMBs) and conjugation conditions were optimized. A high capture efficiency was obtained with 0.4 mg of IMBs, an immunoreaction time of 20 min, and a separation time of 1 min without wash. Under optimal conditions, the capture efficiency (CE) for 10-10 CFU/mL of was higher than 91.46%. The IMBs showed high specificity even with a high constant number (10 CFU/mL) of , , and . The CE of IMBs against at concentrations from 10 to 10 CFU/mL ranged from 78.70 to 94.77% for lettuce and 60.0 to 73.27% for milk samples. This IMS can be an appropriate selection for combining with bacterial detection method or efficient isolation procedure for from foods.
开发了一种使用抗体包被的 Dynabeads Protein G 的免疫磁分离方法，用于从生菜和全脂牛奶中特异性、高效地分离[具体细菌名称未给出]。对免疫磁珠（IMBs）的用量和偶联条件进行了优化。使用 0.4 mg 的 IMBs、20 分钟的免疫反应时间和 1 分钟的分离时间（无需洗涤）可获得较高的捕获效率。在最佳条件下，对于 10 - 10 CFU/mL 的[具体细菌名称未给出]，捕获效率（CE）高于 91.46%。即使存在高浓度（10 CFU/mL）的[其他细菌名称未给出]、[其他细菌名称未给出]和[其他细菌名称未给出]，IMBs 仍显示出高特异性。对于生菜样品，IMBs 针对浓度为 10 至 10 CFU/mL 的[具体细菌名称未给出]的 CE 范围为 78.70%至 94.77%，对于牛奶样品则为 60.0%至 73.27%。这种免疫磁分离方法可作为与细菌检测方法结合使用的合适选择，或作为从食品中高效分离[具体细菌名称未给出]的程序。
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