Microbiology, Fermentation and Biotechnology Division, Central Institute of Fisheries Technology, Cochin, India.
J Appl Microbiol. 2009 Feb;106(2):515-24. doi: 10.1111/j.1365-2672.2008.04020.x.
To determine the distribution of Salmonella serovars in seafood and to examine the intraserovar genetic variations in Salmonella enterica subsp. enterica serovar Rissen and Salmonella Weltevreden by polymerase chain reaction (PCR)-ribotyping and enterobacterial repetitive intergenic consensus (ERIC)-PCR methods.
A total of 417 seafood samples collected over 2003-2006 from fishing harbours and fish markets of Cochin (India) was studied for presence of Salmonella serovars. Seafood samples were analysed for the presence of Salmonella by Bacteriological Analytical Manual (BAM), U.S. Food & Drug Administration (USFDA) method. The study indicated that 23.2% of the seafood samples were positive for Salmonella and a total of 241 Salmonella isolates comprising of 27 different serovars were isolated from seafood. S. Weltevreden, Salmonella Rissen, Salmonella Typhimurium and Salmonella Derby were found to be the most predominant serovars in seafood. PCR-ribotypes and ERIC-PCR profiles showed multiple genotypic profiles for S. Rissen and S. Weltevreden in seafood and the level of discrimination indices obtained was at 0.974 for S. Rissen and 0.988 for S. Weltevreden, respectively.
The study highlighted the major Salmonella serovars in the seafood of Cochin (India) and molecular fingerprinting pattern revealed genetic variation among S. Rissen and S. Weltevreden.
Widespread occurrence of Salmonella contamination in seafood and multiple clones of S. Rissen and S. Weltevreden detected in seafood, thus, indicated the diverse routes of Salmonella contamination in seafood.
确定海产品中沙门氏菌血清型的分布，并通过聚合酶链反应（PCR）-核糖体分型和肠细菌重复基因间一致性（ERIC）-PCR 方法研究沙门氏菌肠道亚种沙门氏菌 Rissen 和沙门氏菌 Weltevreden 种内的遗传变异。
2003 年至 2006 年期间，从印度科钦的渔港和鱼市场共采集了 417 份海产品样本，研究沙门氏菌血清型的存在情况。采用美国食品药品监督管理局（USFDA）方法和细菌分析手册（BAM）对海产品样本进行沙门氏菌检测。研究表明，23.2%的海产品样本呈沙门氏菌阳性，从海产品中分离出 241 株沙门氏菌，包括 27 种不同血清型。沙门氏菌 Weltevreden、沙门氏菌 Rissen、沙门氏菌 Typhimurium 和沙门氏菌 Derby 是海产品中最主要的血清型。PCR-核糖体分型和 ERIC-PCR 图谱显示，海产品中存在多种 S. Rissen 和 S. Weltevreden 的基因型，S. Rissen 和 S. Weltevreden 的分辨指数分别为 0.974 和 0.988。
该研究强调了印度科钦海产品中的主要沙门氏菌血清型，分子指纹图谱显示 S. Rissen 和 S. Weltevreden 之间存在遗传变异。
沙门氏菌污染在海产品中的广泛存在以及在海产品中检测到的 S. Rissen 和 S. Weltevreden 的多个克隆表明，沙门氏菌在海产品中的污染途径多种多样。
