Lopatek Magdalena, Wieczorek Kinga, Osek Jacek
Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland.
Foods. 2022 Nov 4;11(21):3521. doi: 10.3390/foods11213521.
The aim of this study was to evaluate the microbiological contamination of raw bivalve molluscan shellfish (BMS) available on the Polish market and determinate the antimicrobial resistance of the obtained isolates. A total of 1000 mollusc samples were tested for the presence of spp., , , and using the ISO standard methods. Additionally, the bacterial isolates' susceptibility to antimicrobials was determined using the minimum inhibitory concentration (MIC) method. The obtained results showed that spp. was detected in 31 (3.1%) samples, and 51.6% of the bacterial isolates were classified as Typhimurium. A total of 74.2% of the isolates were sensitive to all antimicrobial agents, whereas three isolates were multiresistant. was isolated from 18 (1.8%) BMS, and the isolates belonged to serogroups IIa, IIb, and IVb. Most of them were resistant to ceftriaxone (77.8%) and oxacillin (55.6%). was present in 24.2% BMS. These isolates were mainly resistant to ampicillin (77.3%) and streptomycin (64.0%). Moreover, 15.2% of the bivalve molluscs were contaminated with . Most isolates belonging to this species were resistant to penicillin (84.9%). A total of 60 (6.0%) bivalve molluscs were contaminated with more than one pathogen simultaneously. In addition, the tested bacteria were more likely to be identified during the warmer period (53.9%) compared to the samples analyzed in colder months (35.7%). The obtained results indicate that raw bivalve molluscs from the Polish market are frequently contaminated with bacterial foodborne pathogens, which may be resistant to antimicrobials.
本研究的目的是评估波兰市场上可获得的生双壳贝类软体动物（BMS）的微生物污染情况，并确定所获得分离株的抗菌药物耐药性。使用ISO标准方法对总共1000份软体动物样本进行了检测，以确定是否存在 spp.、 、 和 。此外，使用最低抑菌浓度（MIC）法确定细菌分离株对抗菌药物的敏感性。所得结果表明，在31份（3.1%）样本中检测到 spp.，51.6%的细菌分离株被归类为鼠伤寒沙门氏菌。总共74.2%的 分离株对所有抗菌药物敏感，而有3株分离株具有多重耐药性。从18份（1.8%）BMS中分离出 ，分离株属于血清群IIa、IIb和IVb。其中大多数对头孢曲松（77.8%）和苯唑西林（55.6%）耐药。24.2%的BMS中存在 。这些分离株主要对氨苄西林（77.3%）和链霉素（64.0%）耐药。此外，15.2%的双壳贝类软体动物被 污染。属于该物种的大多数分离株对青霉素（84.9%）耐药。总共60份（6.0%）双壳贝类软体动物同时被一种以上病原体污染。此外，与在较冷月份分析的样本（35.7%）相比，在温暖时期检测到的细菌更有可能被鉴定出来（占53.9%）。所得结果表明，波兰市场上的生双壳贝类软体动物经常被食源性细菌病原体污染，这些病原体可能对抗菌药物耐药。
