Shabarinath S, Sanath Kumar H, Khushiramani Rekha, Karunasagar Indrani, Karunasagar Iddya
Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences, University, College of Fisheries, Mangalore-575 002, India.
Int J Food Microbiol. 2007 Mar 10;114(2):227-33. doi: 10.1016/j.ijfoodmicro.2006.09.012. Epub 2006 Nov 30.
The prevalence of Salmonella in seafood samples collected from the southwest coast of India was studied by conventional culture and by a DNA based molecular technique, polymerase chain reaction (PCR). While conventional culture techniques detected Salmonella in only 20 out of the 100 samples analyzed, direct enrichment lysate PCR detected 52 as positive for Salmonella. A set of three different PCR primers viz., hns, invA and invE were used. It was observed that hns primer detected Salmonella in a significantly higher number of samples. Fourteen out of nineteen isolates belonged to serovar S. enterica Weltevreden. S. Weltevreden isolates were genotyped yielding 4 different patterns both by RAPD and ERIC-PCR but when combined, the overall results discriminated the isolates of S. Weltevreden into 6 different types. This suggests that genetically diverse Salmonella Weltevreden are prevalent in seafood.
采用传统培养方法和基于DNA的分子技术——聚合酶链反应（PCR），对从印度西南海岸采集的海鲜样本中沙门氏菌的流行情况进行了研究。传统培养技术在分析的100个样本中仅检测到20个样本含有沙门氏菌，而直接富集裂解物PCR检测出52个样本呈沙门氏菌阳性。使用了一组三种不同的PCR引物，即hns、invA和invE。观察到hns引物检测出沙门氏菌的样本数量显著更多。19株分离株中有14株属于肠炎沙门氏菌韦尔泰弗里登血清型。韦尔泰弗里登沙门氏菌分离株通过随机扩增多态性DNA（RAPD）和肠杆菌基因间重复一致序列聚合酶链反应（ERIC-PCR）进行基因分型，产生了4种不同模式，但综合起来，总体结果将韦尔泰弗里登沙门氏菌分离株分为6种不同类型。这表明基因多样的韦尔泰弗里登沙门氏菌在海鲜中普遍存在。
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