Centre for Epidemiology and Risk Analysis, Veterinary Laboratories Agency (VLA), Addlestone, Surrey, UK.
J Appl Microbiol. 2009 Feb;106(2):613-23. doi: 10.1111/j.1365-2672.2008.04033.x.
To investigate the factors influencing the presence and burden of Escherichia coli O157 in farm wastes.
Wastes from six cattle farms were screened for the presence and concentration of E. coli O157 and E. coli on three occasions over a year and waste management data were collected. Sixty-three of 878 (7.1%) samples were positive for verocytotoxigenic Escherichia coli O157 and 664/875 (75.9%) for E. coli with detectable levels greater in fresh waste than in stored waste, pasture or dirty water.
The turning/stirring of stored waste and the use of more than one store (allowing longer storage times) reduced the proportion of E. coli O157 positive samples. The presence of E. coli O157 significantly reduced from a high prevalence found in fresh faeces and stored waste to lower proportions in dirty water and pasture samples. Escherichia coli O157 was only detected on pasture when waste was spread from contaminated stores the day before sampling. A high prevalence of positive E. coli O157 samples were detected when cattle were re-housed.
These findings help to support the importance of treating and storing farm waste, as well as providing evidence for the level of dilution of E. coli O157 from fresh waste to recently spread pastures.
研究影响农场废物中大肠杆菌 O157 存在和负担的因素。
在一年中的三个不同时间点，对来自六个奶牛场的废物进行了筛选，以检测大肠杆菌 O157 和大肠杆菌的存在和浓度，并收集了废物管理数据。在 878 个样本中，有 63 个（7.1%）样本为产志贺毒素的大肠杆菌 O157 阳性，664 个（75.9%）样本为大肠杆菌，新鲜废物中的检出水平高于储存废物、牧场或脏水中的检出水平。
翻动/搅拌储存废物以及使用多个储存器（允许更长的储存时间）减少了大肠杆菌 O157 阳性样本的比例。在新鲜粪便和储存废物中发现的大肠杆菌 O157 高流行率显著降低，在脏水和牧场样本中的比例较低。当废物在前一天从污染的储存器中散布时，仅在牧场上检测到大肠杆菌 O157。当牛重新安置时，检测到大量阳性大肠杆菌 O157 样本。
这些发现有助于支持处理和储存农场废物的重要性，并为从新鲜废物到最近散布的牧场的大肠杆菌 O157 稀释水平提供证据。
