聚合氯化铝增强污水中脊髓灰质炎病毒和 f2 噬菌体的浓缩效率。

Polyaluminum chloride-enhanced concentration efficiency of poliovirus and f2 phage from sewage water.

机构信息

Institute of Environment and Health, Tianjin, P.R. China.

出版信息

J Appl Microbiol. 2009 Feb;106(2):660-5. doi: 10.1111/j.1365-2672.2008.04041.x.

DOI:10.1111/j.1365-2672.2008.04041.x

PMID:19200330

Abstract

AIMS

To enhance the recovery of f(2) bacteriophage and poliovirus by an established method based on the adsorption to and elution from positively-charged Al(OH)(3)-treated silica gel.

METHODS AND RESULTS

Polyaluminum Chloride (PAC) was added to water samples to neutralize the negatively charged materials, which can reduce virus recovery by providing a competing adsorption mode on the media surface. Using this improved process (PAC 30 mg l(-1), pH 6.5, temperature 20 approximately 30 degrees C), the recoveries of Poliovirus I and f(2) from small-volume sewage (100 ml) were 110.76 +/- 36.0% and 92.06 +/- 8.65%, respectively (P < 0.05 vs. traditional methods). Recovery from a 20-L volume of sewage averaged 85.65 +/- 4.43% for f(2) and 88.73 +/- 9.76% for poliovirus, significantly higher than the recoveries in the traditional methods (P < 0.05).

CONCLUSIONS

PAC could enhance concentration efficiency of poliovirus and f(2) phage from sewage water.

SIGNIFICANCE AND IMPACT OF THE STUDY

This method should significantly improve the recovery of viruses from sewage.

摘要

目的

通过一种基于正电荷处理的硅胶对带负电荷的物质的吸附和洗脱的既定方法，提高 f(2)噬菌体和脊髓灰质炎病毒的回收率。

方法和结果

向水样中添加聚合氯化铝（PAC）以中和带负电荷的物质，这可以通过在介质表面提供竞争吸附模式来降低病毒的回收率。使用这种改进的工艺（PAC30mg/L，pH6.5，温度 20-30°C），从小体积污水（100ml）中回收脊髓灰质炎病毒 I 和 f(2)的回收率分别为 110.76 +/- 36.0%和 92.06 +/- 8.65%（P < 0.05 与传统方法相比）。从 20 升体积的污水中，f(2)的回收率平均为 85.65 +/- 4.43%，脊髓灰质炎病毒的回收率为 88.73 +/- 9.76%，明显高于传统方法的回收率（P < 0.05）。