Hettinga K A, van Valenberg H J F, Lam T J G M, van Hooijdonk A C M
Dairy Science and Technology Group, Wageningen University and Research Centre, 6700EV Wageningen, The Netherlands.
Vet Microbiol. 2009 Jun 12;137(3-4):384-7. doi: 10.1016/j.vetmic.2009.01.016. Epub 2009 Jan 14.
The possibility to detect mastitis pathogens based on their volatile metabolites was previously studied. Because the origin of the metabolites is unknown, the formation of volatile metabolites by five mastitis pathogens inoculated in milk of healthy cows was studied. The volatile metabolites from inoculated samples were compared to those of mastitis milk samples from which the inoculated pathogens were isolated. Most metabolites formed in the inoculated samples were similar to the metabolites formed in mastitis samples, both in presence and in amount. Prediction by a neural network showed that the similarity between the inoculated samples and mastitis samples was sufficient for correct prediction of the pathogen in the inoculated sample. The main difference between the inoculated samples and the mastitis samples was the absence of ethyl esters of free fatty acids in inoculated samples. This could be explained by disturbance of the milk-blood barrier, allowing the transfer of esterase from the cows' blood to the milk in cows with mastitis.
先前已对基于乳腺炎病原体挥发性代谢物进行检测的可能性展开研究。由于代谢物的来源不明，因此研究了接种于健康奶牛乳汁中的五种乳腺炎病原体挥发性代谢物的形成情况。将接种样本的挥发性代谢物与分离出接种病原体的乳腺炎乳汁样本的代谢物进行了比较。接种样本中形成的大多数代谢物在种类和数量上均与乳腺炎样本中形成的代谢物相似。神经网络预测表明，接种样本与乳腺炎样本之间的相似性足以正确预测接种样本中的病原体。接种样本与乳腺炎样本之间的主要差异在于接种样本中不存在游离脂肪酸乙酯。这可以通过乳腺炎奶牛的血乳屏障受到干扰来解释，该干扰使得酯酶能够从奶牛血液转移至乳汁中。
