Bauvy Chantal, Meijer Alfred J, Codogno Patrice
INSERM U756, Université Paris-Sud 11, Châtenay-Malabry, France.
Methods Enzymol. 2009;452:47-61. doi: 10.1016/S0076-6879(08)03604-5.
Macroautophagy is a three-step process: (1) autophagosomes form and mature, (2) the autophagosomes fuse with lysosomes, and (3) the autophagic cargo is degraded in the lysosomes. It is this lysosomal degradation of the autophagic cargo that constitutes the autophagic flux. As in the case of metabolic pathways, the steady-state concentration of the intermediary autophagic structures alone is insufficient for investigating the flux. Assaying the degradation of long-lived proteins as described in this chapter is one of the methods that can be used to measure autophagic flux.
（1）自噬体形成并成熟，（2）自噬体与溶酶体融合，（3）自噬货物在溶酶体中被降解。正是自噬货物的这种溶酶体降解构成了自噬通量。与代谢途径的情况一样，仅中间自噬结构的稳态浓度不足以研究通量。如本章所述，测定长寿蛋白的降解是可用于测量自噬通量的方法之一。
