Zhang Tingting, Yu Gang, Wang Bin, Fiedler Heidelore, Huang Jun, Deng Shubo
POPs Research Centre, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
POPs Research Centre, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Chemosphere. 2009 May;75(5):649-653. doi: 10.1016/j.chemosphere.2008.12.064. Epub 2009 Feb 6.
In this preliminary study, the bioanalytical characterization of dioxin-like activity in the sludge of all the nine municipal sewage treatment plants from Beijing city was studied using chemically activated luciferase gene expression (CALUX) assay. The influence of heating period in winter, sewage and sludge treatment processes on the occurrence of dioxin-like activity was also discussed. For the use of clean coal and natural gas, heating did not have significant influence on the occurrence level of PCDD/Fs and dioxin-like-PCBs in this study. Anaerobic-aerobic-anoxic sewage treatment process did not show a good performance in the reduction of dioxin-like activity which is useful in the removal of chemical oxygen demand (COD) and nitrogen. Anaerobic digestion of sludge increased the concentration of PCDD/Fs from 30.1 to 68.3pgCALUX-TEQg(-1)d.w., and total dioxin-like activity from 32.2 to 69.3pgCALUX-TEQg(-1)d.w. This cost-effective and quick test is useful for large developing countries like China in monitoring programs to obtain baseline data about the scale of contamination caused by dioxin-like activity. It is also a useful component in the monitoring capacity building of dioxins.
在这项初步研究中，采用化学激活荧光素酶基因表达（CALUX）分析法，对北京市9座城市污水处理厂污泥中二噁英类活性的生物分析特性进行了研究。同时还讨论了冬季供暖期、污水及污泥处理工艺对二噁英类活性产生情况的影响。在本研究中，对于使用清洁煤和天然气的情况，供暖对多氯二苯并对二噁英/多氯二苯并呋喃（PCDD/Fs）及二噁英类多氯联苯（dioxin-like-PCBs）的产生水平没有显著影响。厌氧-好氧-缺氧污水处理工艺在降低二噁英类活性方面表现不佳，而该工艺在去除化学需氧量（COD）和氮方面却很有效。污泥厌氧消化使PCDD/Fs的浓度从30.1增加到68.3pgCALUX-TEQg（-1）干重，二噁英类总活性从32.2增加到69.3pgCALUX-TEQg（-1）干重。这种经济高效且快速的检测方法，对于像中国这样较大的发展中国家在监测项目中获取有关二噁英类活性造成的污染规模的基线数据很有用。它也是二噁英监测能力建设中的一个有用组成部分。
