Gosselin E, Gorez M, Voué M, Denis O, Conti J, Popovic N, Van Cauwenberge A, Noel E, De Coninck J
Centre de Recherche en Modélisation Moléculaire, Université de Mons-Hainaut, Place du Parc, 20, B-7000 Mons, Belgium.
Biosens Bioelectron. 2009 Apr 15;24(8):2554-8. doi: 10.1016/j.bios.2009.01.001. Epub 2009 Jan 14.
We report experimental results concerning the detection of 2,4-dinitrophenol, under its free form or coupled to human serum albumin using Fourier transform infrared spectroscopy-based sensors. Competitive immunoreactions were carried out using several anti-dinitrophenol monoclonal antibodies. Comparison with enzyme-linked immunosorbent assays in competition is given for standard operating conditions. FTIR detection limits are comparable to those obtained by ELISA. The limits of detection are about 5-15 ng/mL for the coupled DNP. Using the LO-DNP61 antibody, a detection limit of congruent with 5 ng/mL was also estimated for the free DNP molecules but is much higher for the other antibodies.
我们报告了关于使用基于傅里叶变换红外光谱的传感器检测游离形式或与人血清白蛋白偶联的2,4-二硝基苯酚的实验结果。使用几种抗二硝基苯酚单克隆抗体进行了竞争性免疫反应。给出了在标准操作条件下与竞争性酶联免疫吸附测定法的比较。傅里叶变换红外光谱检测限与酶联免疫吸附测定法获得的检测限相当。偶联二硝基苯酚的检测限约为5-15 ng/mL。使用LO-DNP61抗体，游离二硝基苯酚分子的检测限估计也为5 ng/mL，但其他抗体的检测限要高得多。
