Chen Zai-Gang, Tang Dian-Yong
Department of Biology, Chongqing Three Gorges University, Chongqing 400400, People's Republic of China.
Bioprocess Biosyst Eng. 2007 Jul;30(4):243-9. doi: 10.1007/s00449-007-0120-5.
A new quartz crystal microbalance immunoassay for the detection of carcinoembryonic antigen (CEA) was developed by means of immobilizing anti-CEA onto magnetic CoFe2O4/SiO2 composite nanoparticles-functionalized biomimetic interface. Under optimal conditions, the frequency shift was proportional to the CEA concentration in the range of 2.5-55 ng/mL with a detection limit of 0.5 ng/mL at a signal-to-noise ratio of 3. Moreover, the immunosensor system showed an acceptable reproducibility and stability. Clinical serum specimens were assayed with this method, and the results were in acceptable agreement with those obtained from ELISA. Compared with the conventional ELISA assay, the proposed immunoassay system was simple and rapid without multiple labeling and separation steps. Importantly, the developed immunoassay protocol could be further extended for the determination of other antigens.
通过将抗癌胚抗原（CEA）固定在磁性CoFe2O4/SiO2复合纳米颗粒功能化的仿生界面上，开发了一种用于检测癌胚抗原（CEA）的新型石英晶体微天平免疫分析法。在最佳条件下，频率变化与CEA浓度在2.5 - 55 ng/mL范围内成正比，在信噪比为3时检测限为0.5 ng/mL。此外，免疫传感器系统显示出可接受的重现性和稳定性。用该方法检测临床血清标本，结果与酶联免疫吸附测定（ELISA）法所得结果具有可接受的一致性。与传统的ELISA检测相比，所提出的免疫分析系统简单快速，无需多次标记和分离步骤。重要的是，所开发的免疫分析方案可进一步扩展用于其他抗原的测定。
