Perinpanayagam Hiran, Al-Rabeah Ebtehal
Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Apr;107(4):590-6. doi: 10.1016/j.tripleo.2008.12.005. Epub 2009 Feb 6.
Periradicular healing involves osteoblasts that are dependent on the Runt-related transcription factor 2 (Runx2). The purpose of this study was to determine if mineral trioxide aggregate (MTA) root-end filling materials support Runx2 expression in osteoblasts.
Human alveolar bone cells were grown on alternative formulations of MTA. Cell-surface interactions were visualized by scanning electron microscopy. Gene expression was examined by reverse-transcription polymerase chain reaction and Western blot analysis.
Cells attached to and spread out on MTA surfaces within 24 hours and formed a collagenous matrix overlay within 1 week of growth. Runx2 expression increased from low levels in the 24-hour cultures to an abundance during 2 weeks of growth and differentiation on MTA surfaces and on tissue culture plastic controls. The cells responded similarly to ProRoot, Tooth-Colored MTA, and MTA mixed with local anesthetic solution.
Mineral trioxide aggregate materials support cell attachment and Runx2 expression in osteoblasts.
根尖周愈合涉及依赖于 runt 相关转录因子 2（Runx2）的成骨细胞。本研究的目的是确定三氧化矿物凝聚体（MTA）根充材料是否支持成骨细胞中 Runx2 的表达。
将人牙槽骨细胞培养在 MTA 的不同配方上。通过扫描电子显微镜观察细胞表面相互作用。通过逆转录聚合酶链反应和蛋白质印迹分析检测基因表达。
细胞在 24 小时内附着并铺展在 MTA 表面，并在生长 1 周内形成胶原基质覆盖层。Runx2 的表达从 24 小时培养物中的低水平增加到在 MTA 表面和组织培养塑料对照上生长和分化 2 周期间的丰富水平。细胞对 ProRoot、牙色 MTA 以及与局部麻醉溶液混合的 MTA 的反应相似。
三氧化矿物凝聚体材料支持成骨细胞的细胞附着和 Runx2 表达。
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