Min Kyung-San, Yang Seong-Hak, Kim Eun-Cheol
Department of Conservative Dentistry, School of Dentistry, Wonkwang University, Iksan, South Korea.
J Endod. 2009 Jun;35(6):847-51. doi: 10.1016/j.joen.2009.03.014.
The current study investigated the effectiveness of enamel matrix derivative (EMD) and mineral trioxide aggregate (MTA) in inducing the differentiation of human dental pulp cells (HDPCs) into odontoblast-like cells in vitro.
The effects of MTA and EMD on odontoblastic differentiation were indexed by alkaline phosphatase (ALP) activity and the expression of odontoblastic/osteoblastic markers, as determined by reverse-transcription polymerase chain reaction analysis. Mineralization was also evaluated by the staining of calcium deposits with Alizarin red. Cells were treated with a combination of MTA and EMD or with MTA alone.
Compared with MTA-treated cells on day 3, MTA/EMD-treated cells exhibited significantly greater increases in ALP activity and in dentin sialophosphoprotein and bone sialoprotein expression. Mineralization was significantly greater on day 7 in MTA/EMD-treated cells than in MTA-treated cells.
These results suggest that a combination of MTA and EMD promotes more rapid differentiation in HDPCs than MTA alone and that this combination is thus a potential pulp-capping agent.
本研究调查了釉基质衍生物（EMD）和三氧化矿物凝聚体（MTA）在体外诱导人牙髓细胞（HDPCs）分化为成牙本质样细胞的有效性。
通过碱性磷酸酶（ALP）活性以及通过逆转录聚合酶链反应分析测定的成牙本质细胞/成骨细胞标志物的表达，来衡量MTA和EMD对成牙本质细胞分化的影响。还用茜素红对钙沉积进行染色来评估矿化情况。细胞分别用MTA和EMD的组合或单独用MTA处理。
与第3天用MTA处理的细胞相比，用MTA/EMD处理的细胞在ALP活性以及牙本质涎磷蛋白和骨涎蛋白表达方面的增加显著更大。在第7天，用MTA/EMD处理的细胞的矿化程度明显高于用MTA处理的细胞。
这些结果表明，MTA和EMD的组合比单独使用MTA能促进HDPCs更快地分化，因此这种组合是一种潜在的盖髓剂。
