Zhu Q, Haglund R, Safavi K E, Spangberg L S
Department of Endodontology, School of Dental Medicine, University of Connecticut Health Center 06030-1715, USA.
J Endod. 2000 Jul;26(7):404-6. doi: 10.1097/00004770-200007000-00006.
Adhesion of human osteoblasts to root-end filling materials (mineral trioxide aggregate (MTA), IRM, composite, and amalgam) was observed by scanning electron microscopy. Root-end filling materials were inserted into 96-well flat-bottomed plates and condensed to disks of approximately 1 mm thick and the same diameter as the wells. After the disks were set, they were placed in the bottom of Nunc four-well culture plates at one disk per well. Then human osteoblasts were seeded into the wells at 1.5 x 10(5) cells per well. After 1 day in culture the disks of root-end filling materials along with cells grown on their surface were examined with a scanning electron microscopy. Results showed that osteoblasts attached and spread on MTA and composite by forming a monolayer. Osteoblasts also attached on amalgam, but with few cells spreading. In the presence of IRM, osteoblasts appeared rounded with no spreading. These results indicate that osteoblasts have a favorable response to MTA and composite resin compared with IRM and amalgam.
通过扫描电子显微镜观察人成骨细胞与根尖充填材料（矿物三氧化物凝聚体（MTA）、IRM、复合树脂和汞合金）的黏附情况。将根尖充填材料插入96孔平底培养板中，压实成厚度约为1 mm且直径与培养板孔相同的圆盘。圆盘凝固后，将其置于Nunc四孔培养板底部，每孔一个圆盘。然后将人成骨细胞以每孔1.5×10⁵个细胞的密度接种到孔中。培养1天后，用扫描电子显微镜检查根尖充填材料圆盘及其表面生长的细胞。结果显示，成骨细胞通过形成单层附着并铺展在MTA和复合树脂上。成骨细胞也附着在汞合金上，但铺展的细胞很少。在IRM存在的情况下，成骨细胞呈圆形，没有铺展。这些结果表明，与IRM和汞合金相比，成骨细胞对MTA和复合树脂有良好的反应。
