García Celia Carrillo, Diago Miguel Peñarrocha, Mira Berta García, Sebastián José Vicente Bagán, Sempere Francisco Vera
Oral Surgery and Implantology, Valencia University Medical and Dental School, Valencia, Spain.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Apr;107(4):e43-6. doi: 10.1016/j.tripleo.2008.12.002. Epub 2009 Feb 6.
This study attempted to clarify whether differential immunocytokeratin expression occurs in cystic and epithelialized noncystic lesions.
From January 2003 to June 2004, periapical surgery was carried out on 70 teeth, collecting 70 histologic samples. Serial sections of original histologic slices were prepared for immunostaining. The antibodies used were against cytokeratins CK7, AE1, AE3, AE1-3, and CAM52. The intensity of staining was evaluated as follows: 0 = no cells stained; 1 = weak staining (11%-25%); 2 = moderate staining (26%-75%); and 3 = intense staining (>or=76%).
Ten epithelized granulomas and 6 cysts were included. Intense staining was seen for AE1 and AE1-3 in the 2 types of lesion; staining with AE3 was moderate for epithelized granulomas and cysts. CK7 demonstrated a light reactivity in the outermost epithelial layer. CAM52 showed weak staining for the epithelized granuloma and moderate for the cyst.
Cysts and epithelized granulomas presented the same cytokeratin expression, though with marked differences in distribution and degree of staining of cytokeratins. This immunochemical technique is interesting for the determination of the histologic type and the prognosis, but may not be clinically practicable.
本研究试图阐明在囊性病变和上皮化非囊性病变中是否存在不同的免疫细胞角蛋白表达。
从2003年1月至2004年6月，对70颗牙齿进行根尖手术，收集70份组织学样本。对原始组织切片制备连续切片用于免疫染色。所使用的抗体针对细胞角蛋白CK7、AE1、AE3、AE1 - 3和CAM52。染色强度评估如下：0 = 无细胞染色；1 = 弱染色（11% - 25%）；2 = 中度染色（26% - 75%）；3 = 强染色（≥76%）。
纳入10个上皮化肉芽肿和6个囊肿。在这两种病变类型中均可见AE1和AE1 - 3的强染色；上皮化肉芽肿和囊肿中AE3染色为中度。CK7在最外层上皮层显示轻度反应性。CAM52在上皮化肉芽肿中显示弱染色，在囊肿中显示中度染色。
囊肿和上皮化肉芽肿呈现相同的细胞角蛋白表达，尽管在细胞角蛋白的分布和染色程度上存在明显差异。这种免疫化学技术对于确定组织学类型和预后很有意义，但可能在临床上不实用。
