Delzangles B, Boy-Lefevre M L, Forest N
Department of Endodontics, University Paris VII, France.
J Endod. 1997 Sep;23(9):565-8. doi: 10.1016/S0099-2399(06)81122-2.
Indirect immunofluorescence was used for the localization of the primary collagens, fibronectin and laminin. Specimens were extracted from untreated teeth with periapical lesions from patients 20 to 30 years of age. An histological examination enabled the differentiation of granulomas and cysts, and 5 microns sections were used for the indirect immunofluorescence procedure. Antibodies against Type I, Type III, and Type V collagen and for fibronectin and laminin were obtained from glycoproteins of human cells. The antibody against Type IV collagen was prepared from Type IV collagen of beef retina. All the glycoproteins investigated were expressed in apical lesions. The intensity of the immunostaining appeared more positive at the external area compared with the center of the lesion. The type IV collagen was specific for the basement membrane of cysts. The immunofluorescence reactions of fibronectin and of laminin were similar in intensity in both granulomas and cysts.
采用间接免疫荧光法对Ⅰ型、Ⅲ型和Ⅴ型胶原蛋白、纤连蛋白和层粘连蛋白进行定位。从20至30岁患有根尖周病变的未经治疗的牙齿中提取标本。组织学检查能够区分肉芽肿和囊肿，5微米厚的切片用于间接免疫荧光检测。针对Ⅰ型、Ⅲ型和Ⅴ型胶原蛋白以及纤连蛋白和层粘连蛋白的抗体取自人细胞糖蛋白。抗Ⅳ型胶原蛋白抗体由牛视网膜Ⅳ型胶原蛋白制备。所有研究的糖蛋白均在根尖病变中表达。与病变中心相比，免疫染色强度在病变外部区域显得更阳性。Ⅳ型胶原蛋白对囊肿的基底膜具有特异性。纤连蛋白和层粘连蛋白的免疫荧光反应在肉芽肿和囊肿中的强度相似。
