Kilvington Simon, Lonnen James
Department of Infection, Immunity & Inflammation, University of Leicester, Leicester, United Kingdom.
Cont Lens Anterior Eye. 2009 Apr;32(2):73-7. doi: 10.1016/j.clae.2008.12.008. Epub 2009 Feb 6.
To compare the antimicrobial efficacy of commercial contact lens solutions when used according to the manufacturers' recommended regimens with two types of silicone hydrogel lenses.
Four multipurpose contact lens care solutions were examined, representing manufacturer recommended regimens of "rub & rinse", "no rub, rinse" or "no rub, no rinse". Test organisms were Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Fusarium solani, Candida albicans and Acanthamoeba castellanii (trophozoites and cysts). Organisms, in the presence of organic soil, were inoculated on to Acuvue Oasys or Air Optix lenses and subjected to the solution manufacturer's recommended regimen. The number of surviving organisms on the lenses and in the soak solution was enumerated in accordance with ISO 14729.
ISO 14729 dictates that for a given organism the combined average number of surviving microbes from the lenses and disinfectant soaking solution must be <or=10 colony forming units (cfu)/ml. By this criterion, only Complete Easy Rub ("rub & rinse" regimen) gave satisfactory results for all bacteria, fungi and Acanthamoeba with both lens types. Solutions employing "no rub, rinse" were less satisfactory but significantly better than "no rub, no rinse". Significant differences were found in organism survival on the lenses with greater numbers remaining on the Air Optix compared to Oasys (p<0.01-0.0001).
The findings of this study demonstrate that the use of a manual rubbing step is more effective than rinsing or soaking alone in removing pathogenic microbes from silicone hydrogel lenses. Accordingly, it would seem prudent to recommend that contact lens care systems include a rub step as part of the hygiene regimen.
比较按照制造商推荐方案使用时，市售隐形眼镜护理液对两种类型硅水凝胶镜片的抗菌效果。
检测了四种多功能隐形眼镜护理液，分别代表制造商推荐的“揉搓冲洗”“不揉搓、冲洗”或“不揉搓、不冲洗”方案。测试微生物包括铜绿假单胞菌、粘质沙雷氏菌、金黄色葡萄球菌、茄病镰刀菌、白色念珠菌和卡氏棘阿米巴（滋养体和包囊）。将存在有机污垢情况下的微生物接种到酷柏欧舒适氧或爱尔康视康水润型镜片上，并按照护理液制造商推荐的方案进行处理。根据ISO 14729对镜片上和浸泡液中存活微生物的数量进行计数。
ISO 14729规定，对于给定的微生物，镜片和消毒浸泡液中存活微生物的综合平均数量必须≤10菌落形成单位（cfu）/毫升。按照这一标准，只有全能舒适护理液（“揉搓冲洗”方案）对两种镜片类型的所有细菌、真菌和棘阿米巴均给出了满意结果。采用“不揉搓、冲洗”方案的护理液效果欠佳，但明显优于“不揉搓、不冲洗”方案。在镜片上的微生物存活情况方面发现了显著差异，与欧舒适相比，视康水润型镜片上残留的微生物数量更多（p<0.01 - 0.0001）。
本研究结果表明，在从硅水凝胶镜片上去除致病微生物方面，手动揉搓步骤比单独冲洗或浸泡更有效。因此，建议将揉搓步骤纳入隐形眼镜护理系统的卫生方案中似乎是审慎之举。
