Tripathi Sudipta, Bruch David, Gatto Louis A, Kittur Dilip S
Department of Surgery, The State University of New York Upstate Medical University, Syracuse, New York 13210, USA.
J Surg Res. 2009 Jun 1;154(1):85-90. doi: 10.1016/j.jss.2008.05.005. Epub 2008 Jun 2.
The current immunosuppressive drugs are successful in prolonging allograft survival but fail to achieve transplantation tolerance or prevent chronic rejection. Consequently, there is ongoing research to develop novel combinatorial therapies that are more efficacious in prolonging allograft survival as well as induce tolerance toward the transplanted organ. The present study aims to study the efficacy of green tea extract (GTE) in combination with low dose cyclosporine A (CyA) in prolonging allograft survival in mice. Numerous studies have reported the anti-inflammatory and immunomodulatory properties of GTE and its various catechin components. GTE is also known to attenuate CyA induced nephrotoxicity. Therefore, we hypothesized that GTE alone or in combination with CyA will prolong graft survival. Our study demonstrates that GTE in combination with low dose CyA significantly prolongs graft survival as well as increase the production of immunosuppressive cytokine, IL-10. GTE also decreases CyA induced high TGF-beta production, which is incriminated in CyA induced nephrotoxicity. We also observed that GTE inhibits both nonspecific and antigen-specific proliferation of T cells in vitro. These results indicate the potential of GTE as an adjunctive therapy in combination with CyA to prolong allograft survival and to reduce CyA induced nephrotoxicity.
目前的免疫抑制药物在延长同种异体移植物存活时间方面取得了成功，但未能实现移植耐受或预防慢性排斥反应。因此，正在进行研究以开发新型联合疗法，这些疗法在延长同种异体移植物存活时间以及诱导对移植器官的耐受方面更有效。本研究旨在研究绿茶提取物（GTE）与低剂量环孢素A（CyA）联合使用在延长小鼠同种异体移植物存活时间方面的疗效。许多研究报道了GTE及其各种儿茶素成分的抗炎和免疫调节特性。GTE还已知可减轻CyA诱导的肾毒性。因此，我们假设单独使用GTE或与CyA联合使用将延长移植物存活时间。我们的研究表明，GTE与低剂量CyA联合使用可显著延长移植物存活时间，并增加免疫抑制细胞因子IL-10的产生。GTE还可降低CyA诱导的高TGF-β产生，而TGF-β与CyA诱导的肾毒性有关。我们还观察到，GTE在体外抑制T细胞的非特异性和抗原特异性增殖。这些结果表明，GTE作为与CyA联合使用的辅助疗法，在延长同种异体移植物存活时间和降低CyA诱导的肾毒性方面具有潜力。
