Rokas Antonis, Abbot Patrick
Department of Biological Sciences, Vanderbilt University, VU Station B 35-1634, Nashville, TN 37235, USA.
Trends Ecol Evol. 2009 Apr;24(4):192-200. doi: 10.1016/j.tree.2008.11.004. Epub 2009 Feb 7.
Next-generation DNA sequencing technologies can generate unprecedented amounts of genomic data, even for non-model organisms. Here we describe how these new technologies have facilitated recent key advances in ecology and evolutionary biology, and highlight several outstanding ecological and evolutionary questions that are distinctly suited to the innovations they provide. Importantly, using these technologies to their full potential requires careful experimental design and critical consideration of several caveats associated with them. Although several significant challenges remain to be resolved before the integration of next-generation sequencing technologies into single-investigator research programs, we argue that they will soon transform ecology and evolution by fundamentally changing the ranges and types of questions that can be addressed.
下一代DNA测序技术能够生成数量空前的基因组数据，即使是针对非模式生物。在此我们描述这些新技术如何推动了生态学和进化生物学领域近期的关键进展，并强调了几个特别适合于它们所提供创新的突出的生态学和进化问题。重要的是，要充分发挥这些技术的潜力需要精心的实验设计以及对与其相关的若干注意事项进行审慎考虑。尽管在将下一代测序技术整合到单研究者研究项目之前仍有若干重大挑战有待解决，但我们认为它们将很快通过从根本上改变能够解决的问题的范围和类型来变革生态学和进化研究。
