Broers C A, Stumm C K, Vogels G D
Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
J Protozool. 1991 Sep-Oct;38(5):507-11. doi: 10.1111/j.1550-7408.1991.tb04825.x.
The strain N of Trimyema compressum, an anaerobic free-living ciliate, was cultivated axenically in a medium containing a buffered salt solution, yeast extract, trypticase, and glutathione. Dead bacteria were indispensable as food; a culture of the ciliate together with heat-killed Klebsiella pneumoniae has been established for more than one year. In the medium described, the ciliates grow to a higher cell density than in cultures with living bacteria as food. During the process of axenization, a nonmethanogenic bacterial endosymbiont was lost. In the microbodies of T. compressum, hydrogenase could be localized by the technique of indirect immunofluorescence.
扁裸藻（Trimyema compressum）的N菌株是一种厌氧的自由生活纤毛虫，在含有缓冲盐溶液、酵母提取物、胰蛋白酶和谷胱甘肽的培养基中进行无菌培养。死细菌作为食物是必不可少的；纤毛虫与热灭活的肺炎克雷伯菌的培养物已经建立了一年多。在所述培养基中，纤毛虫比以活细菌为食物的培养物生长到更高的细胞密度。在无菌培养过程中，一种非产甲烷细菌内共生体丢失了。在扁裸藻的微体中，可通过间接免疫荧光技术定位氢化酶。
