在钾离子和钠离子存在的情况下，凝血酶DNA适配体鸟嘌呤四链体稳定性的圆二色性研究。
A circular dichroism study of the stability of guanine quadruplexes of thrombin DNA aptamers at presence of K+ and Na+ ions.
作者信息
Poniková Slavomíra, Antalík Marián, Hianik Tibor
机构信息
Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, 842 48 Bratislava, Slovakia.
出版信息
Gen Physiol Biophys. 2008 Dec;27(4):271-7.
The effect of K+ and Na+ on the properties of DNA aptamers that selective binds to fibrinogen (FIBRI) and heparin (HEPA) exosites of human alpha-thrombin was studied by circular dichroism (CD). The complexes of FIBRI-K+ were slightly more stable than HEPA-K+. However, lower stability was observed for HEPA-K+ at presence of Na+ in comparison with FIBRI-K+. The analysis of CD melting curves suggests differences in thermal stability of both aptamers at presence of K+. The melting temperatures (Tm) and changes in van't Hoff enthalpy for HEPA-K+ complexes were lower in comparison with those for FIBRI-K+. With increasing HEPA concentration the Tm value increased, but Tm did not change with increasing FIBRI concentration. This suggests formation of HEPA aggregates, while FIBRI aptamers are in monomeric form.
通过圆二色性（CD）研究了钾离子（K⁺）和钠离子（Na⁺）对选择性结合人α-凝血酶纤维蛋白原（FIBRI）和肝素（HEPA）外位点的DNA适配体性质的影响。FIBRI-K⁺复合物比HEPA-K⁺略稳定。然而，与FIBRI-K⁺相比，在有Na⁺存在时，HEPA-K⁺的稳定性较低。CD熔解曲线分析表明，在有K⁺存在时，两种适配体的热稳定性存在差异。与FIBRI-K⁺复合物相比，HEPA-K⁺复合物的熔解温度（Tm）和范特霍夫焓变较低。随着HEPA浓度的增加，Tm值升高，但随着FIBRI浓度的增加，Tm没有变化。这表明形成了HEPA聚集体，而FIBRI适配体呈单体形式。
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