基于适体探针和分子光开关复合物的无标记双重 DNA 序列和钾离子检测。
Label-free dual assay of DNA sequences and potassium ions using an aptamer probe and a molecular light switch complex.
机构信息
Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
出版信息
Chem Commun (Camb). 2009 Dec 21(47):7419-21. doi: 10.1039/b915994k. Epub 2009 Oct 22.
DOI:10.1039/b915994k
PMID:20024248
Abstract
A homogeneous assay is reported using Ru(phen)(2)(dppz)(2+) and a K(+)-binding aptamer for the selective and sensitive detection of a target oligonucleotide and potassium ions, based on reduction in fluorescence emission according to the formation of the G-quadruplex structure from the aptamer in the presence of K(+).
摘要
报道了一种均相分析方法，使用 Ru(phen)(2)(dppz)(2+) 和与钾离子结合的适体，通过适体在钾离子存在下形成 G-四链体结构，根据荧光发射的减少，选择性和灵敏地检测靶寡核苷酸和钾离子。
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