Lee Mak-Soon, Kim Yangha
Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Korea.
Biosci Biotechnol Biochem. 2009 Feb;73(2):434-6. doi: 10.1271/bbb.80563. Epub 2009 Feb 7.
In this study, we investigated the effects of green tea (-)-epigallocatechin-3-gallate (EGCG) on the mRNA level and promoter activity of uncoupling protein 2 (UCP2), a mitochondrial membrane transporter that regulates energy expenditure and thermogenesis in 3T3-L1 adipocytes. EGCG up-regulated the UCP2 mRNA level in a dose-dependent manner. UCP2 promoter activity was significantly stimulated by EGCG treatment, to an extent similar to that seen in mRNA expression. These results suggest that expression of UCP2 gene is directly regulated by green tea EGCG, which is mediated through the transcriptional activation of its proximal promoter.
在本研究中，我们调查了绿茶（-）-表没食子儿茶素-3-没食子酸酯（EGCG）对解偶联蛋白2（UCP2）的mRNA水平和启动子活性的影响。UCP2是一种线粒体膜转运蛋白，可调节3T3-L1脂肪细胞中的能量消耗和产热。EGCG以剂量依赖的方式上调UCP2 mRNA水平。EGCG处理显著刺激了UCP2启动子活性，其程度与mRNA表达中所见相似。这些结果表明，UCP2基因的表达直接受绿茶EGCG调控，这是通过其近端启动子的转录激活介导的。
