Beale David J, Porter Nichola A, Roddick Felicity A
School of Applied Sciences (Applied Chemistry), RMIT University, GPO Box 2476V, Melbourne 3001, Australia.
Talanta. 2009 Apr 30;78(2):342-7. doi: 10.1016/j.talanta.2008.11.033. Epub 2008 Nov 30.
Atrazine is a triazine herbicide which contains two secondary aliphatic amine groups. Previous studies have shown that aliphatic amines react with tris(2,2'-bipyridyl)ruthenium(III) to produce chemiluminescence. This paper describes the application of tris(2,2'-bipyridyl)ruthenium(III) to the detection of atrazine and related triazine herbicides in water by flow injection chemiluminescence analysis. The optimised experimental conditions were determined to be: sample and carrier flow rates of 4.6 mL min(-1), sample at pH 9 buffered with 50mM borax, and reagent concentration of 1mM tris(2,2'-bipyridyl)ruthenium(III) in 20mM H(2)SO(4) (pH 1). Under these conditions, the logarithm of the chemiluminescence intensity versus concentration was linear in the range of 2.15-2150 microg L(-1) for samples in MilliQ water, and the limit of detection of atrazine in water was determined to be 1.3+/-0.1 microg L(-1). Validation of the method was performed using direct injection HPLC. The presence of natural organic matter (NOM) significantly increased the chemiluminescence, masking the signal generated by atrazine. Isolating the target analyte via solid phase extraction (SPE) prior to analysis removed this interference and concentrated the samples, resulting in a greatly improved sensitivity with a detection limit of 14+/-2 ng L(-1).
莠去津是一种含有两个仲脂肪胺基团的三嗪类除草剂。先前的研究表明，脂肪胺与三（2,2'-联吡啶）钌（III）反应会产生化学发光。本文描述了三（2,2'-联吡啶）钌（III）在流动注射化学发光分析中用于检测水中莠去津及相关三嗪类除草剂的应用。优化后的实验条件确定为：样品和载液流速为4.6 mL min⁻¹，样品用50 mM硼砂缓冲至pH 9，试剂浓度为1 mM三（2,2'-联吡啶）钌（III）于20 mM H₂SO₄（pH 1）中。在这些条件下，对于超纯水样品，化学发光强度的对数与浓度在2.15 - 2150 μg L⁻¹范围内呈线性，水中莠去津的检测限确定为1.3 ± 0.1 μg L⁻¹。使用直接进样高效液相色谱法对该方法进行了验证。天然有机物（NOM）的存在显著增强了化学发光，掩盖了莠去津产生的信号。在分析前通过固相萃取（SPE）分离目标分析物消除了这种干扰并浓缩了样品，从而大大提高了灵敏度，检测限为14 ± 2 ng L⁻¹。
