基于N-(氨丁基)-N-乙基异鲁米诺标记和金纳米粒子放大的夹心型电化学发光免疫传感器。
Sandwich-type electrochemiluminescence immunosensor based on N-(aminobutyl)-N-ethylisoluminol labeling and gold nanoparticle amplification.
作者信息
Tian Dayong, Duan Chunfeng, Wang Wei, Li Na, Zhang Hao, Cui Hua, Lu Yingyu
机构信息
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
出版信息
Talanta. 2009 Apr 30;78(2):399-404. doi: 10.1016/j.talanta.2008.11.037. Epub 2008 Dec 3.
A novel electrochemiluminescence (ECL) sandwich-type immunosensor for human immunoglobulin G (hIgG) on a gold nanoparticle modified electrode was developed by using N-(aminobutyl)-N-ethylisoluminol (ABEI) labeling. The primary antibody, goat-anti-human IgG was first immobilized on a gold nanoparticle modified electrode, then the antigen (human IgG) and the ABEI-labeled second antibody was conjugated successively to form a sandwich-type immunocomplex. ECL was carried out with a double-step potential in carbonate buffer solution (CBS) containing 1.5mM H(2)O(2). The ECL intensity increased linearly with the concentration of hIgG over the range 5.0-100 ng/mL. The limit of detection was 1.68 ng/mL (S/N=3). The relative standard deviation was 3.79% at 60 ng/mL (n=9). The present immunosensor is simple and sensitive. It has been successfully applied to the detection of hIgG in human serums.
通过使用N-(氨丁基)-N-乙基异鲁米诺（ABEI）标记，在金纳米颗粒修饰电极上开发了一种用于检测人免疫球蛋白G（hIgG）的新型电化学发光（ECL）夹心型免疫传感器。首先将一抗山羊抗人IgG固定在金纳米颗粒修饰电极上，然后依次结合抗原（人IgG）和ABEI标记的二抗，形成夹心型免疫复合物。在含有1.5mM H₂O₂的碳酸盐缓冲溶液（CBS）中通过双步电位进行ECL检测。ECL强度在5.0 - 100 ng/mL范围内随hIgG浓度呈线性增加。检测限为1.68 ng/mL（S/N = 3）。在60 ng/mL时相对标准偏差为3.79%（n = 9）。该免疫传感器简单且灵敏，已成功应用于人血清中hIgG的检测。
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