CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, PR China.
Biosens Bioelectron. 2011 Sep 15;27(1):18-24. doi: 10.1016/j.bios.2011.05.022. Epub 2011 Jun 21.
A novel nanoparticle-based electrochemiluminescence (ECL) immunosensor was designed for highly sensitive and selective detection of human cardiac troponin I (cTnI), an important Acute Myocardial Infarction (AMI)-related biomarker, by using N-(aminobutyl)-N-(ethylisoluminol)-functionalized gold nanoparticles (ABEI-AuNPs) as labels. ABEI-AuNPs were successfully synthesized via a simple seed growth method. A great number of luminescence molecules ABEI as stabilizers were coated on the surface of the AuNPs, which exhibited better ECL activities than previously reported luminol functionalized gold nanoparticles. ABEI-AuNPs were used as new ECL labels to build bio-probes by conjugation with secondary antibodies, which showed good ECL activity, immunological activity, and stability. Another kind of AuNPs functionalized with streptavidin was modified on the electrode surface for biotinylated antibodies capture through the specific interaction of biotin/streptavidin and enhancing the electrical connectivity. By combining with the novel ECL labels and amplification of AuNPs and biotin-streptavidin system, a high sensitive sandwich-type electrochemiluminescence immunoassay was developed for detecting human cTnI with a low detection limit of 2 pg/mL. The immunosensor showed good precision, acceptable stability and reproducibility and could be used for the detection of cTnI in real samples, which was of great potential application in clinical analysis. Importantly, the sensitive detection would have far more diagnostic value than would absolute measurements during the early stage of AMI.
一种新型基于纳米粒子的电化学发光（ECL）免疫传感器被设计用于高度灵敏和选择性地检测人心肌肌钙蛋白 I（cTnI），cTnI 是一种重要的急性心肌梗死（AMI）相关生物标志物，该传感器通过使用 N-（氨丁基）-N-（乙基异鲁米诺）功能化金纳米粒子（ABEI-AuNPs）作为标记物。ABEI-AuNPs 通过简单的种子生长法成功合成。大量的发光分子 ABEI 作为稳定剂被包覆在 AuNPs 的表面上，其 ECL 活性比以前报道的鲁米诺功能化金纳米粒子要好。ABEI-AuNPs 被用作生物探针的新型 ECL 标记物，通过与二级抗体的共轭反应，其表现出良好的 ECL 活性、免疫活性和稳定性。另一种通过静电吸附作用将链霉亲和素功能化的 AuNPs 修饰在电极表面，用于通过生物素/链霉亲和素的特异性相互作用捕获生物素化的抗体，并增强电子连通性。通过结合新型 ECL 标记物和 AuNPs 的放大以及生物素-链霉亲和素系统，开发了一种高灵敏度的夹心型电化学发光免疫分析方法，用于检测人 cTnI，其检测限低至 2 pg/mL。该免疫传感器具有良好的精密度、可接受的稳定性和重现性，可用于实际样品中 cTnI 的检测，在临床分析中具有很大的应用潜力。重要的是，与绝对测量相比，在 AMI 的早期阶段，灵敏的检测将具有更大的诊断价值。
