Brne P, Lim Y-P, Podgornik A, Barut M, Pihlar B, Strancar A
BIA Separations d.o.o., Teslova 30, 1000 Ljubljana, Slovenia.
J Chromatogr A. 2009 Mar 27;1216(13):2658-63. doi: 10.1016/j.chroma.2008.11.005. Epub 2008 Nov 7.
Convective interaction media (CIM; BIA Separations) monoliths are attractive stationary phases for use in affinity chromatography because they enable fast affinity binding, which is a consequence of convectively enhanced mass transport. This work focuses on the development of novel CIM hydrazide (HZ) monoliths for the oriented immobilization of antibodies. Adipic acid dihydrazide (AADH) was covalently bound to CIM epoxy monoliths to gain hydrazide groups on the monolith surface. Two different antibodies were afterwards immobilized to hydrazide functionalized monolithic columns and prepared columns were tested for their selectivity. One column was further tested for the dynamic binding capacity.
对流相互作用介质（CIM；BIA分离公司）整体柱是用于亲和色谱的有吸引力的固定相，因为它们能够实现快速亲和结合，这是对流增强传质的结果。这项工作专注于开发用于抗体定向固定的新型CIM酰肼（HZ）整体柱。己二酸二酰肼（AADH）共价结合到CIM环氧整体柱上，以在整体柱表面获得酰肼基团。随后将两种不同的抗体固定到酰肼功能化的整体柱上，并对制备的柱子进行选择性测试。对其中一根柱子进一步测试其动态结合容量。
