Jiang Zhiliang, Liang Aihui, Li Yan, Wei Xiaoling
Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin 541004, China.
IEEE Trans Nanobioscience. 2008 Dec;7(4):276-83. doi: 10.1109/TNB.2008.2011860.
A novel immunonanogold-catalytic Cu(2)O-enhanced resonance scattering (RS) spectral assay is reported, based on nanogold modified rabbit anti-penicillin G (RAPG) antibody, its catalytic enhanced effect on the slow particle reaction between Cu(II) and glucose, and the RS effect of (Au) (nucleus)(Cu(2)O) (shell) complex particle at 608 nm. As a model, we used nanogold in size of 9 nm to label RAPG to obtain an immunonanogold probe (AuRAPG) for penicillin G (PG). The immunoreactions between the probe and PG took place in phosphate-citric acid buffer solutions. After centrifugation, the excess AuRAPG in the supernatant was used to catalyze the particle reaction to amplify the RS signal. With the addition of PG, the concentration of AuRAPG in the supernatant reduced linearly, which leaded the I(608 nm) to decrease accordingly. The decreased RS intensity was proportional to the PG concentration in the range of 0.09-21.6 ng x mL(-1), with a detection limit (DL) of 0.01 ng x mL(-1) PG. The assay was used to determine trace PG in raw milk sample, with the recovery between 100.6% and 109.2%.
报道了一种基于纳米金修饰兔抗青霉素G（RAPG）抗体、其对铜（II）与葡萄糖之间慢粒子反应的催化增强作用以及（金）（核）（氧化亚铜）（壳）复合粒子在608nm处的共振散射（RS）效应的新型免疫纳米金催化氧化亚铜增强共振散射光谱分析方法。作为模型，我们使用9nm大小的纳米金标记RAPG以获得用于青霉素G（PG）的免疫纳米金探针（AuRAPG）。探针与PG之间的免疫反应在磷酸盐 - 柠檬酸缓冲溶液中进行。离心后，上清液中过量的AuRAPG用于催化粒子反应以放大RS信号。随着PG的加入，上清液中AuRAPG的浓度呈线性降低，这导致608nm处的吸光度（I(608 nm)）相应降低。在0.09 - 21.6 ng x mL(-1)范围内，降低的RS强度与PG浓度成正比，PG的检测限（DL）为0.01 ng x mL(-1)。该分析方法用于测定生乳样品中的痕量PG，回收率在100.6%至109.2%之间。
