Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Education Ministry, Guangxi Key Laboratory of Environmental Engineering and Protection Assessment, Guangxi Normal University, Guilin, China.
Bioprocess Biosyst Eng. 2011 May;34(4):499-504. doi: 10.1007/s00449-010-0494-7. Epub 2010 Dec 9.
Nanogold of 10 nm was used to label carcinoembryonic antigen antibody (CEAAb) to prepare a probe (Au-CEAAb) for carcinoembryonic antigen (CEA). In a Na₂HPO₄-NaH₂PO₄ buffer solution of pH 6.8, CEA reacted with Au-CEAAb to form a big Au-CEAAb-CEA immunocomplex that can be removed by centrifugation. The unreacted Au-CEAAb in the centrifugal supernatant exhibited catalytic effect on the Cu₂O particle reaction, and the Cu₂O particles displayed a resonance scattering (RS) peak at 602 nm. When CEA increased, the RS intensity at 602 nm decreased, and the decreased RS intensity (ΔI (602 nm)) was linear to CEA concentration (C (CEA)) in the range of 0.02-12 ng mL(-1), with the regression equation of ΔI (602 nm) = 27.1 C (CEA) + 3.3, correlation coefficient of 0.9978 and detection limit of 3 pg mL(-1) CEA. The proposed method was applied to detect CEA in real samples, with satisfactory results.
纳米金被用于标记癌胚抗原抗体（CEAAb），以制备用于癌胚抗原（CEA）的探针（Au-CEAAb）。在 pH 值为 6.8 的 Na₂HPO₄-NaH₂PO₄ 缓冲溶液中，CEA 与 Au-CEAAb 反应形成可以通过离心去除的大的 Au-CEAAb-CEA 免疫复合物。离心上清液中未反应的 Au-CEAAb 对 Cu₂O 颗粒反应表现出催化作用，并且 Cu₂O 颗粒在 602nm 处显示共振散射（RS）峰。当 CEA 增加时，在 602nm 处的 RS 强度降低，并且降低的 RS 强度（ΔI（602nm））与 0.02-12ng mL（-1）范围内的 CEA 浓度（C（CEA））呈线性关系，回归方程为ΔI（602nm）=27.1C（CEA）+3.3，相关系数为 0.9978，CEA 的检测限为 3pg mL（-1）。该方法用于检测实际样品中的 CEA，结果令人满意。
