Harju Anni M, Venäläinen Martti, Laakso Tapio, Saranpää Pekka
Finnish Forest Research Institute, Punkaharju Research Unit, FI-58450 Punkaharju, Finland.
Tree Physiol. 2009 Jan;29(1):19-25. doi: 10.1093/treephys/tpn006. Epub 2008 Dec 5.
In this greenhouse experiment, 3-year-old Scots pine (Pinus sylvestris L.) seedlings were wounded by drilling holes through the stem. In the xylem next to the wound, the concentration of resin acids (RAC) increased, and the production of extractives typical for heartwood (stilbenes) and knotwood (stilbenes and lignans) of mature trees was induced. The induced stilbenes were pinosylvin (PS) and pinosylvin monomethyl ether (PSM), and the lignans nortrachelogenin (NTG) and matairesinol (MR). There was positive phenotypic correlation between concentrations of the different extractives. Except for the RAC, the extractive concentrations showed no correlation with the size of the seedlings. The treated seedlings belonged to half-sib families, which enabled the estimation of the genetic parameters for the response variables. The proportion of heritable variation (heritability, h(2)) in the concentration of PS, NTG and MR varied between 0.71 and 1.03, whereas for PSM and RAC the heritability was lower (0.35 and 0.31). Genetic correlation was significant between PS and PSM (r = 0.55, P = 0.018), and between NTG and MR (r = 0.50, P = 0.033). Heritabilities were also estimated on the basis of the regression of the offspring on their mothers h(2)(0P). These estimates were assessed for the concentration of PS, PSM and RAC in the wound response area of the seedlings and correspondingly in the heartwood of their mothers. The heritability was highest for the concentration of PS h(2)(0P). The findings of this study support the suggestion that the wounding of Scots pine seedlings may facilitate the development of an early testing method for breeding heartwood durability.
在这个温室实验中，对3年生的欧洲赤松（Pinus sylvestris L.）幼苗的茎干打孔造成创伤。在伤口附近的木质部中，树脂酸（RAC）的浓度增加，并且诱导了成熟树木心材（芪类）和节木（芪类和木脂素）典型提取物的产生。诱导产生的芪类物质是松二氢菲（PS）和松二氢菲单甲醚（PSM），木脂素是去甲落叶松脂素（NTG）和罗汉松脂素（MR）。不同提取物的浓度之间存在正表型相关性。除了RAC外，提取物浓度与幼苗大小无关。处理过的幼苗属于半同胞家系，这使得能够估计响应变量的遗传参数。PS、NTG和MR浓度的遗传变异比例（遗传力，h²）在0.71至1.03之间，而PSM和RAC 的遗传力较低（分别为0.35和0.31）。PS和PSM之间以及NTG和MR之间的遗传相关性显著（r = 0.55，P = 0.018；r = 0.50，P = 0.033）。还根据子代对其母本的回归估计了遗传力h²(0P)。对幼苗伤口反应区域以及相应的其母本心材中PS、PSM和RAC的浓度进行了这些估计。PS浓度的遗传力h²(0P)最高。本研究结果支持以下建议：欧洲赤松幼苗创伤可能有助于开发一种用于培育心材耐久性的早期测试方法。
