Vaezy S, Clark J I
Centre for Bioengineering WD-12, University of Washington, Seattle 98195.
J Microsc. 1991 Jul;163(Pt 1):85-94. doi: 10.1111/j.1365-2818.1991.tb03162.x.
Cellular microstructure observed by scanning transmission electron microscopy (STEM) was analysed using Fourier methods. Fourier components of the density fluctuations in the sclera and the cornea of the human eye were quantified. The results show that the Fourier components responsible for the opacity of the sclera have sizes of the order of the wavelength of visible light. In the sclera, approximately 54% of the spectral energy of the density fluctuation falls in the range of 200-1100 nm (scattering range). In the cornea, approximately 24% of the total spectral energy falls in this range. The predominant Fourier components of the density fluctuations in the opaque sclera are approximately 300 nm in wavelength, whereas those of the transparent cornea are approximately 80 nm in wavelength. This method will be useful in quantitative analysis of microstructural changes accompanying biological phenomena such as normal development of transparency in the human lens, and abnormal loss of transparency during cataract formation.
利用傅里叶方法分析了通过扫描透射电子显微镜（STEM）观察到的细胞微观结构。对人眼巩膜和角膜中密度波动的傅里叶分量进行了量化。结果表明，导致巩膜不透明的傅里叶分量大小约为可见光波长量级。在巩膜中，密度波动的光谱能量约54%落在200 - 1100 nm范围内（散射范围）。在角膜中，总光谱能量约24%落在该范围内。不透明巩膜中密度波动的主要傅里叶分量波长约为300 nm，而透明角膜的主要傅里叶分量波长约为80 nm。该方法将有助于对伴随生物现象的微观结构变化进行定量分析，如人晶状体透明度的正常发育以及白内障形成过程中透明度的异常丧失。
