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利用二维傅里叶分析对亚硒酸盐性白内障晶状体细胞微观结构进行定量分析。

Quantitative analysis of the lens cell microstructure in selenite cataract using a two-dimensional Fourier analysis.

作者信息

Vaezy S, Clark J I, Clark J M

机构信息

Department of Biological Structure SM-20, University of Washington, Seattle 98195, USA.

出版信息

Exp Eye Res. 1995 Mar;60(3):245-55. doi: 10.1016/s0014-4835(05)80107-1.

DOI:10.1016/s0014-4835(05)80107-1
PMID:7789405
Abstract

Using two-dimensional (2-D) Fourier methods, we analysed the cellular microstructure of three rat lenses: normal transparent, selenite-induced cataractous and selenite-treated plus a phase separation inhibitor (PSI) to prevent cataract. 2-D Fourier analysis of electron micrographs of the lens cells quantified the dimensions of the spatial fluctuations in electron density of the lens cell microstructure. The 2-D Fourier spectra of the transparent normal and PSI-treated lens cells were remarkably similar while those of the opaque selenite-treated lens cells were dramatically different. In the opaque cells the contributions of large Fourier components (larger than half the wavelength of light) in the 2-D Fourier spectra were much greater than in the transparent cells. The results of the 2-D Fourier analysis of electron micrographs are consistent with the theory of transparency of the eye.

摘要

我们使用二维(2-D)傅里叶方法,分析了三只大鼠晶状体的细胞微观结构:正常透明的、亚硒酸盐诱导的白内障以及经亚硒酸盐处理并添加相分离抑制剂(PSI)以预防白内障的。对晶状体细胞电子显微照片进行二维傅里叶分析,量化了晶状体细胞微观结构中电子密度空间波动的尺寸。透明的正常晶状体细胞和经PSI处理的晶状体细胞的二维傅里叶光谱非常相似,而不透明的经亚硒酸盐处理的晶状体细胞的二维傅里叶光谱则有显著差异。在不透明细胞中,二维傅里叶光谱中较大傅里叶分量(大于光波长的一半)的贡献远大于透明细胞。对电子显微照片进行二维傅里叶分析的结果与眼睛透明度理论一致。

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Quantitative analysis of the lens cell microstructure in selenite cataract using a two-dimensional Fourier analysis.利用二维傅里叶分析对亚硒酸盐性白内障晶状体细胞微观结构进行定量分析。
Exp Eye Res. 1995 Mar;60(3):245-55. doi: 10.1016/s0014-4835(05)80107-1.
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Exp Eye Res. 2008 Feb;86(2):434-44. doi: 10.1016/j.exer.2007.11.018. Epub 2007 Dec 5.
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Characterization of the cellular microstructure of ocular lens using 2D power law analysis.
Ann Biomed Eng. 1995 Jul-Aug;23(4):482-90. doi: 10.1007/BF02584448.