Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, U.K.
Evol Bioinform Online. 2008 Jun 13;4:203-16. doi: 10.4137/ebo.s743.
beta-propeller domains composed of WD repeats are highly ubiquitous and typically used as multi-site docking platforms to coordinate and integrate the activities of groups of proteins. Here, we have used extensive homology modelling of the WD40-repeat family of seven-bladed beta-propellers coupled with subsequent structural classification and clustering of these models to define subfamilies of beta-propellers with common structural, and probable, functional characteristics. We show that it is possible to assign seven-bladed WD beta-propeller proteins into functionally different groups based on the information gained from homology modelling. We examine general structural diversity within the WD40-repeat family of seven-bladed beta-propellers and demonstrate that seven-bladed beta-propellers composed of WD-repeats are structurally distinct from other seven-bladed beta-propellers. We further provide some insights into the multifunctional diversity of the seven-bladed WD beta-propeller surfaces. This report once again reinforces the importance of structural data and the usefulness of homology models in functional classification.
β-类桨叶结构域由 WD 重复序列组成，高度普遍存在，通常用作多部位对接平台，以协调和整合蛋白质组的活性。在这里，我们通过对七叶型β-类桨叶 WD40 重复家族进行广泛的同源建模，然后对这些模型进行结构分类和聚类，从而定义了具有共同结构和可能的功能特征的β-类桨叶亚家族。我们表明，基于同源建模获得的信息，可以根据功能将七叶型 WDβ-类桨叶蛋白分配到不同的组中。我们检查了七叶型 WD40 重复家族β-类桨叶中的一般结构多样性，并证明由 WD 重复序列组成的七叶型β-类桨叶在结构上与其他七叶型β-类桨叶不同。我们进一步提供了对七叶型 WDβ-类桨叶表面多功能多样性的一些见解。本报告再次强调了结构数据的重要性以及同源模型在功能分类中的有用性。
