患支气管肺发育不良的早产儿脐带血中的基质金属蛋白酶-9和金属蛋白酶组织抑制因子-1

MMP-9 and TIMP-1 in the cord blood of premature infants developing BPD.

作者信息

Fukunaga Shinnosuke, Ichiyama Takashi, Maeba Shinji, Okuda Masayuki, Nakata Masahiko, Sugino Norihiro, Furukawa Susumu

机构信息

Department of Pediatrics, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.

出版信息

Pediatr Pulmonol. 2009 Mar;44(3):267-72. doi: 10.1002/ppul.20993.

DOI:10.1002/ppul.20993

PMID:19205055

Abstract

We investigated matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) levels in the cord blood of 29 premature infants who were <30 weeks gestation. One, 8, and 14 infants developed severe, moderate and mild bronchopulmonary dysplasia (BPD), respectively, and 6 did not. MMP-9 and TIMP-1 levels in the cord blood were determined by ELISA. MMP-9/TIMP-1 ratios in the cord blood of infants who developed severe or moderate BPD (n = 9) were significantly higher than those who developed mild BPD or did not develop BPD (n = 20; P = 0.015). Multivariate linear regressions demonstrated that MMP-9 levels and MMP-9/TIMP-1 ratios in the cord blood of the premature infants correlated with the oxygen supplementation period (r = 0.58, P = 0.003 and r = 0.41, P = 0.030, respectively). The MMP-9 levels and MMP-9/TIMP-1 ratios correlated with the severity of maternal chorioamnionitis (both trend P = 0.006). The MMP-9 levels and MMP-9/TIMP-1 ratios in the cord blood may be related to the pathogenesis and severity of BPD and maternal chorioamnionitis.

摘要

我们研究了29例孕周小于30周的早产儿脐带血中基质金属蛋白酶-9（MMP-9）和金属蛋白酶组织抑制剂1（TIMP-1）的水平。分别有1例、8例和14例婴儿发生了重度、中度和轻度支气管肺发育不良（BPD），6例未发生。通过酶联免疫吸附测定法（ELISA）测定脐带血中的MMP-9和TIMP-1水平。发生重度或中度BPD的婴儿（n = 9）脐带血中的MMP-9/TIMP-1比值显著高于发生轻度BPD或未发生BPD的婴儿（n = 20；P = 0.015）。多变量线性回归表明，早产儿脐带血中的MMP-9水平和MMP-9/TIMP-1比值与吸氧时间相关（r = 0.58，P = 0.003和r = 0.41，P = 0.030）。MMP-9水平和MMP-9/TIMP-1比值与产妇绒毛膜羊膜炎的严重程度相关（趋势P均为0.006）。脐带血中的MMP-9水平和MMP-9/TIMP-1比值可能与BPD及产妇绒毛膜羊膜炎的发病机制和严重程度有关。