Pinto J, Check J H
The University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, Cooper Hospital/University Medical Center, Department of Obstetrics and Gynecology, Camden, NJ, USA.
Clin Exp Obstet Gynecol. 2008;35(4):252-4.
To determine if fertilization and embryo cleavage can be achieved by artificial oocyte activation in circumstances of repeated failed fertilization with sperm that have an acrosome.
A woman with three IVF cycles with intracytoplasmic sperm injection (ICSI) failed to fertilize any eggs. The sperm had severe oligoasthenoteratozoospermia with no sperm with normal morphology. In the fourth IVF cycle fertilization was evaluated by performing ICSI with the husband's sperm and egg activation with calcium ionophore, ICSI with the husband's sperm without artificial oocyte activation, and ICSI with donor sperm.
Five mature oocytes were retrieved. Of the four eggs having ICSI with the husband's sperm only one of the two activated by calcium ionophore fertilized and resulted in a cleaved day 3 embryo. Interestingly, the one egg fertilized by donor sperm did not fertilize.
The data could be consistent with conclusions that in some cases the failure to fertilize may be related to an oocyte activation factor/receptor problem in the oocyte that can be overcome by the use of calcium ionophore.
确定在使用具有顶体的精子反复受精失败的情况下，通过人工卵母细胞激活是否能够实现受精和胚胎分裂。
一名女性进行了三个周期的卵胞浆内单精子注射（ICSI）体外受精，均未成功使任何卵子受精。其丈夫的精子存在严重少弱畸精子症，无正常形态的精子。在第四个体外受精周期中，通过用丈夫的精子进行ICSI并使用钙离子载体激活卵子、用丈夫的精子进行ICSI但不进行人工卵母细胞激活以及用供体精子进行ICSI来评估受精情况。
共获取了5个成熟卵子。在使用丈夫的精子进行ICSI的4个卵子中，仅2个经钙离子载体激活的卵子中有1个受精，并形成了第3天的分裂胚胎。有趣的是，经供体精子受精的1个卵子未受精。
这些数据可能与以下结论一致，即在某些情况下，受精失败可能与卵母细胞中的卵母细胞激活因子/受体问题有关，而使用钙离子载体可以克服这一问题。
