Hunter S M, Berrang M E, Meinersmann R J, Harrison M A
U.S. Department of Agriculture, Agricultural Research Service, Russell Research Center, Athens, Georgia 30605, USA.
J Food Prot. 2009 Jan;72(1):49-54. doi: 10.4315/0362-028x-72.1.49.
Campylobacter jejuni and Campylobacter coli are the most important human enteropathogens among the campylobacters. The objective of this study was to determine how diversity in Campylobacter populations found on chicken carcasses collected from 17 broiler processing plants in the United States is impacted by processing. Genetic diversity was determined for up to four isolates per carcass by sequencing the short variable region (SVR) of the flaA locus. On 70% of Campylobacter-positive carcasses, all isolates were indistinguishable by flaA SVR typing. The genetic diversity of Campylobacter decreased as carcasses proceeded through processing; Campylobacter populations obtained early in processing where carcasses are moved from the kill line to the evisceration line (rehang) were significantly more genetically diverse (P < 0.05) than those from carcasses sampled postchill (diversity indices of 0.9472 and 0.9235, respectively). Certain Campylobacter subtypes were found only at rehang and not at postchill. Other subtypes were found at postchill and not at rehang. These data suggest that some subtypes may not be able to survive processing, whereas others may persist on the carcass or within the equipment despite stressors encountered in the processing environment.
空肠弯曲菌和结肠弯曲菌是弯曲菌属中最重要的人类肠道病原体。本研究的目的是确定在美国17家肉鸡加工厂采集的鸡胴体上发现的弯曲菌种群多样性如何受到加工过程的影响。通过对flaA基因座的短可变区（SVR）进行测序，确定每个胴体多达四个分离株的遗传多样性。在70%的弯曲菌阳性胴体上，所有分离株通过flaA SVR分型无法区分。随着胴体进入加工过程，弯曲菌的遗传多样性降低；在加工早期，即胴体从屠宰线转移到去内脏线（重新悬挂）时获得的弯曲菌种群，其遗传多样性显著高于冷藏后采样的胴体（多样性指数分别为0.9472和0.9235，P < 0.05）。某些弯曲菌亚型仅在重新悬挂时发现，而在冷藏后未发现。其他亚型在冷藏后发现，而在重新悬挂时未发现。这些数据表明，一些亚型可能无法在加工过程中存活，而其他亚型尽管在加工环境中遇到应激源，但可能在胴体上或设备内持续存在。
