Naderpour Masoud, Rad Jafar Soleimani, Ayat Esmail, Mesgari Mehran, Farahani Ramin M, Roshangar Leila, Tubbs R Shane, Shoja Mohammadali M
Department of Otolaryngology, Tabriz University of Medical Sciences, Tabriz, Iran.
Ital J Anat Embryol. 2008 Jul-Sep;113(3):135-42.
Skin wound healing has been the subject of extensive studies and various drugs have been used in an attempt to improve wound healing. There are conflicting data regarding the effects of L-arginine, the substrate of nitric oxide, on wound healing. We examined the 1-week rate of cutaneous wound healing and collagen deposition in three groups of rats who received a (1) L-arginine (2% in drinking water)-supplemented diet from three days before until the seventh day following injury (Group 1), (2) L-arginine-supplemented diet for three days before injury (Group 2), and (3) a standard diet without L-arginine supplementation (Group 3). The wound length and width were measured each day and then the open wound area and cumulative percentage of open wound area reduction were calculated. Wound biopsy samples were examined with Trichrome-Masson stain in a subgroup of animals. Results showed that Group 1 rats had a significantly lower cumulative percentage of open wound area reduction on day 7 compared to other two groups (Mann-Whitney U test, P < 0.05). Relatively higher degrees of wound collagen deposit (day 7) were noted in groups 2 and 3. It may be concluded that L-arginine (2% in water) administered three days before until the seventh day following skin wound induction may diminish the rate of skin wound healing and collagen deposition.
皮肤伤口愈合一直是广泛研究的主题，并且已经使用了各种药物来试图改善伤口愈合。关于一氧化氮的底物L-精氨酸对伤口愈合的影响，存在相互矛盾的数据。我们检查了三组大鼠的皮肤伤口愈合1周率和胶原沉积情况，这三组大鼠分别接受了：(1) 从受伤前三天到受伤后第七天给予补充L-精氨酸（饮用水中含2%）的饮食（第1组）；(2) 在受伤前三天给予补充L-精氨酸的饮食（第2组）；以及(3) 不补充L-精氨酸的标准饮食（第3组）。每天测量伤口的长度和宽度，然后计算开放伤口面积和开放伤口面积减少的累积百分比。在一组亚组动物中，用三色-马森染色法检查伤口活检样本。结果显示，与其他两组相比，第1组大鼠在第7天时开放伤口面积减少的累积百分比显著更低（曼-惠特尼U检验，P < 0.05）。在第2组和第3组中观察到相对较高程度的伤口胶原沉积（第7天）。可以得出结论，在皮肤伤口诱导前三天到诱导后第七天给予L-精氨酸（水中含2%）可能会降低皮肤伤口愈合率和胶原沉积。
