Alves Claudia Cristina, Torrinhas Raquel Susana, Giorgi Ricardo, Brentani Maria Mitzi, Logullo Angela Flavia, Waitzberg Dan Linetzky
Laboratory of Nutrition and Metabolic Surgery of the Digestive System (LIM 35), Gastroenterology Department, University of São Paulo Medical School (FMUSP), São Paulo, Brazil; University of São Paulo, NAPAN, Food and Nutrition Research Center, São Paulo, Brazil.
Int Wound J. 2014 Oct;11(5):533-9. doi: 10.1111/j.1742-481X.2012.01120.x. Epub 2012 Nov 30.
Malnutrition is associated with the delay or failure of healing. We assessed the effect of experimental malnutrition and early enteral feeding with standard diet or diet supplemented with arginine and antioxidants on the levels of mRNA encoding growth factors in acute, open wound healing. Standardised cutaneous dorsal wounds and gastrostomies for enteral feeding were created in malnourished (M, n = 27) and eutrophic control (E, n = 30) Lewis male adult rats. Both M and E rats received isocaloric and isonitrogenous regimens with oral chow and saline (C), standard (S) or supplemented (A) enteral diets. On post-trauma day 7, mRNA levels of growth factor genes were analysed in wound granulation tissue by reverse transcription polymerase chain reaction (RT-PCR). M(C) rats had significantly lower transforming growth factor β(TGF-β1 ) mRNA levels than E(C) rats (2·58 ± 0·83 versus 3·53 ± 0·57, P < 0·01) and in comparison with M(S) and M(A) rats (4·66 ± 2·49 and 4·61 ± 2·11, respectively; P < 0·05). VEGF and KGF-7 mRNA levels were lower in M(A) rats than in E(A) rats (0·74 ± 0·16 versus 1·25 ± 0·66; and 1·07 ± 0·45 versus 1·79 ± 0·89, respectively; P≤ 0·04), but did not differ from levels in E(C) and M(C) animals. In experimental open acute wound healing, previous malnutrition decreased local mRNA levels of TGF-β1 genes, which was minimised by early enteral feeding with standard or supplemented diets.
营养不良与愈合延迟或愈合失败有关。我们评估了实验性营养不良以及用标准饮食或添加精氨酸和抗氧化剂的饮食进行早期肠内喂养对急性开放性伤口愈合中生长因子编码mRNA水平的影响。在营养不良的（M组，n = 27）和营养正常的对照（E组，n = 30）成年雄性Lewis大鼠中制造标准化的背部皮肤伤口和用于肠内喂养的胃造口术。M组和E组大鼠均接受等热量和等氮量的方案，分别给予口服饲料和生理盐水（C组）、标准（S组）或补充（A组）肠内饮食。在创伤后第7天，通过逆转录聚合酶链反应（RT-PCR）分析伤口肉芽组织中生长因子基因的mRNA水平。M（C）组大鼠的转化生长因子β（TGF-β1）mRNA水平显著低于E（C）组大鼠（2.58±0.83对3.53±0.57，P<0.01），且与M（S）组和M（A）组大鼠相比也较低（分别为4.66±2.49和4.61±2.11；P<0.05）。M（A）组大鼠的血管内皮生长因子（VEGF）和角质形成细胞生长因子-7（KGF-7）mRNA水平低于E（A）组大鼠（分别为0.74±0.16对1.25±0.66；以及1.07±0.45对1.79±0.89，P≤0.04），但与E（C）组和M（C）组动物的水平无差异。在实验性开放性急性伤口愈合中，既往营养不良会降低TGF-β1基因的局部mRNA水平，而早期用标准或补充饮食进行肠内喂养可将其降至最低。
