Zhang Hai-Yan, Meng Xin, Du Zhen-Xian, Fang Chang-Qing, Liu Guo-Liang, Wang Hua-Qin, Deng Wei-Wei
Department of Geriatrics, The First Affiliated Hospital, China Medical University, Heping District, 110001 Shenyang, Liaoning Province, People's Republic of China.
Clin Exp Med. 2009 Sep;9(3):207-13. doi: 10.1007/s10238-009-0031-7. Epub 2009 Feb 10.
To investigate the clinical significance of survivin, caspase-3, and vascular endothelial growth factor expression in a subset of thyroid carcinoma and their correlation with prognosis. Sixty-eight cases of thyroid carcinoma (TC), 12 cases of thyroid adenoma (TA) and 10 cases of normal thyroid tissue (NT) were involved in immunohistochemical and real-time RT-PCR analyses for survivin, caspase-3, and VEGF expression. Statistical analyses were performed for differential expression among NT, TA and TC, correlations of their expression with the clinicopathological parameters of TC including histological typing, clinical staging and lymphnode metastasis, and relationship of survivin with caspase-3 or VEGF in TC. We observed higher mRNA expression and positive immunostaining for survivin and VEGF in TC compared with TA and NT, with a significant positive correlation among them and significant correlations with histological typing, clinical staging and lymphnode metastasis in TC, but we could not find any significance of caspase-3 in TC and its significant relationship with survivin expression. Our results indicate that survivin and VEGF are unfavorable molecules for TC evolution and prognosis, and possess positive correlation in TC.
探讨存活素、半胱天冬酶-3和血管内皮生长因子在部分甲状腺癌中的表达及其临床意义，以及它们与预后的相关性。选取68例甲状腺癌（TC）、12例甲状腺腺瘤（TA）和10例正常甲状腺组织（NT），进行存活素、半胱天冬酶-3和血管内皮生长因子表达的免疫组化及实时逆转录聚合酶链反应分析。对NT、TA和TC之间的差异表达、它们的表达与TC临床病理参数（包括组织学类型、临床分期和淋巴结转移）的相关性以及TC中存活素与半胱天冬酶-3或血管内皮生长因子的关系进行统计学分析。我们观察到，与TA和NT相比，TC中存活素和血管内皮生长因子的mRNA表达更高，免疫染色呈阳性，它们之间存在显著正相关，且与TC的组织学类型、临床分期和淋巴结转移显著相关，但未发现半胱天冬酶-3在TC中的任何意义及其与存活素表达的显著关系。我们的结果表明，存活素和血管内皮生长因子是TC进展和预后的不良分子，且在TC中呈正相关。
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