Green Howard J, Burnett M E, D'Arsigny C, Iqbal S, Ouyang J, Webb K A, O'Donnell D E
Department of Kinesiology, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
J Mol Histol. 2009 Feb;40(1):41-51. doi: 10.1007/s10735-009-9211-8. Epub 2009 Feb 11.
Chronic obstructive pulmonary disease (COPD) is known to elicit intrinsic abnormalities in male skeletal muscle. However, it is unclear to what extent these changes occur in women and whether they are fiber-type specific. We investigated fiber-type specific differences in selected histochemical properties in muscle obtained from women with moderate to severe COPD compared to healthy control (CON) women. Tissue was obtained from the vastus lateralis in five COPD patients (age 66.9 +/- 2.6 years; FEV1 = 43 +/- 7%) and eight CON (age 68 +/- 4.9 years; FEV1 = 113 +/- 4.2%). Compared to CON, the distribution (30.6 +/- 5.2 vs. 57.9 +/- 4.6%) and cross sectional area of type I (CSA, 5660 +/- 329 vs. 3586 +/- 257 microm2) and type IIA (2770 +/- 302 vs. 2099 +/- 206 microm2) were lower (P < 0.05) and higher (P < 0.05), respectively, in COPD. Disease state did not alter either the distribution or CSA of the IIA, IIAX or type X subtypes. Although differences were found between fiber types in the number of capillary contacts (n) (I > IIAX, IIX; IIA > IIX) and the capillaries per CSA (microm210(-3)) (I < IIA, IIAX, IIX), no differences were found between CON and COPD. Succinic dehydrogenase activity and sarcoplasmic reticulum (SR) Ca2+-ATPase activity, measured photometrically (OD units), were higher (P < 0.05), and lower (P < 0.05), respectively, in type I compared to the type II fiber subtypes. These properties were not altered with COPD. COPD in females is accompanied by a higher percent of type II fibers, a larger CSA of type I and type IIA fibers, both of which occur in the absence of differences in oxidative potential and the potential for SR Ca2+-sequestration.
已知慢性阻塞性肺疾病（COPD）会引发男性骨骼肌的内在异常。然而，目前尚不清楚这些变化在女性中出现的程度以及它们是否具有纤维类型特异性。我们研究了中度至重度COPD女性与健康对照（CON）女性相比，所选肌肉组织化学特性的纤维类型特异性差异。组织取自5名COPD患者（年龄66.9±2.6岁；第一秒用力呼气容积[FEV1]=43±7%）和8名CON（年龄68±4.9岁；FEV1=113±4.2%）的股外侧肌。与CON相比，COPD患者中I型纤维的分布（30.6±5.2%对57.9±4.6%）和横截面积（CSA，5660±329对3586±257μm²）较低（P<0.05），IIA型纤维的分布和横截面积较高（P<0.05），分别为2770±302对2099±206μm²。疾病状态未改变IIA、IIAX或X型亚型的分布或CSA。尽管在毛细血管接触数量（n）（I>IIAX、IIX；IIA>IIX）和每CSA毛细血管数量（μm²×10⁻³）（I<IIA、IIAX、IIX）方面发现纤维类型之间存在差异，但CON和COPD之间未发现差异。通过光度法（光密度单位）测量的琥珀酸脱氢酶活性和肌浆网（SR）Ca²⁺-ATP酶活性，I型纤维分别高于（P<0.05）和低于（P<0.05）II型纤维亚型。这些特性在COPD患者中未发生改变。女性COPD患者伴有较高比例的II型纤维、较大的I型和IIA型纤维CSA，这两种情况均在氧化电位和SR Ca²⁺-螯合潜力无差异的情况下出现。
