Nativo Paola, Prior Ian A, Brust Mathias
Department of Chemistry, University of Liverpool, Liverpool, United Kingdom.
ACS Nano. 2008 Aug;2(8):1639-44. doi: 10.1021/nn800330a.
Understanding and controlling the interactions between nanoscale objects and living cells is of great importance for arising diagnostic and therapeutic applications of nanoparticles and for nanotoxicology studies. Here we report a detailed transmission electron microscopy (TEM) study of the uptake of ca. 16 nm surface-modified gold nanoparticles by human fibroblast cells (HeLa cells). It is demonstrated that the well-established endosomal route of cellular uptake can be bypassed to a significant extent by controlling the uptake mechanism either via the delivery of the nanoparticles by liposomes or by surface modification of the nanoparticles with so-called cell penetrating peptides (CPPs). Successful nuclear targeting is demonstrated using surface modification with a cocktail of CPPs and a peptide acting as a nuclear localization signal (NLS).
理解和控制纳米级物体与活细胞之间的相互作用，对于推动纳米颗粒的诊断和治疗应用以及纳米毒理学研究具有重要意义。在此，我们报告了一项关于人类成纤维细胞（HeLa细胞）摄取约16 nm表面修饰金纳米颗粒的详细透射电子显微镜（TEM）研究。结果表明，通过脂质体递送纳米颗粒或用所谓的细胞穿透肽（CPP）对纳米颗粒进行表面修饰来控制摄取机制，在很大程度上可以绕过已确立的细胞内吞途径。使用CPP混合物和作为核定位信号（NLS）的肽进行表面修饰，证明了成功的核靶向。
