Fisichella Matthieu, Dabboue Hinda, Bhattacharyya Sanjib, Lelong Gérald, Saboungi Marie-Louise, Warmont Fabienne, Midoux Patrick, Pichon Chantal, Guérin Martine, Hevor Tobias, Salvetat Jean-Paul
Centre de Recherche sur la Matière Divisée-UMR6619 Université d'Orléans-CNRS, 1B rue de la Férollerie, 45071 Orleáns Cedex 2, France.
J Nanosci Nanotechnol. 2010 Apr;10(4):2314-24. doi: 10.1166/jnn.2010.1917.
Mesoporous silica nanoparticles (MSN) were functionalised by aminofluorescein (AMF) with diethylenetriaminepentaacetic acid spacer molecules which provide free carboxylic groups for binding cell-specific ligands such as folate. AMF allowed the exploration of cellular uptake by HeLa cells using confocal microscopy and flow cytometry. The functionalized nanoparticles (MSN-AMF) penetrated efficiently into HeLa cell cytoplasm through a clathrin dependent endocytosis mechanism. The number of endocytosed MSN-AMF was enhanced when using folate as a targeting molecule. Uptake kinetics revealed that most of MSN-AMF were internalized within 4 h of incubation. Moreover, we found that MSN-AMF were capable of escaping the acidic endolysosomal vesicles of HeLa cells. Cytotoxicity studies suggested that these nanoparticles are non-toxic to HeLa cells up to a dose level of 50 microg/ml.
介孔二氧化硅纳米颗粒（MSN）通过氨基荧光素（AMF）与二乙烯三胺五乙酸间隔分子进行功能化，这些间隔分子提供游离羧基以结合细胞特异性配体，如叶酸。AMF使得利用共聚焦显微镜和流式细胞术研究HeLa细胞的细胞摄取成为可能。功能化纳米颗粒（MSN-AMF）通过网格蛋白依赖性内吞机制有效地穿透进入HeLa细胞的细胞质。当使用叶酸作为靶向分子时，内吞的MSN-AMF数量增加。摄取动力学表明，大多数MSN-AMF在孵育4小时内被内化。此外，我们发现MSN-AMF能够从HeLa细胞的酸性内溶酶体囊泡中逃逸。细胞毒性研究表明，这些纳米颗粒在剂量水平高达50微克/毫升时对HeLa细胞无毒。
