Mahmoud Khaled A, Hrapovic Sabahudin, Luong John H T
Biotechnology Research Institute, National Research Council Canada, Montreal, Canada H4P2R2.
ACS Nano. 2008 May;2(5):1051-7. doi: 10.1021/nn8000774.
Picomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM.
已报道了使用二茂铁（Fc）-胃蛋白酶抑制剂修饰的表面对人免疫缺陷病毒1型蛋白酶（HIV-1 PR）进行皮摩尔级电化学检测。金电极表面用金纳米颗粒（AuNP）或硫醇化单壁碳纳米管/金纳米颗粒（SWCNT/AuNP）进行修饰。然后通过拉曼光谱和扫描电子显微镜证实，硫醇末端的Fc-胃蛋白酶抑制剂自组装在这些表面上。通过循环伏安法和电化学阻抗谱研究了Fc-胃蛋白酶抑制剂修饰的底物与HIV-1 PR之间的相互作用。两种电极材料对HIV-1 PR浓度增加均表现出增强的电化学响应，电位向更高值移动，同时总信号强度降低。然而，用硫醇化SWCNTs/AuNPs修饰的传感电极显示出显著的检测灵敏度，估计检测限为0.8 pM。
